螺旋藻和C-藻蓝蛋白可降低小胶质细胞的细胞毒性和炎症相关基因表达。
Spirulina and C-phycocyanin reduce cytotoxicity and inflammation-related genes expression of microglial cells.
作者信息
Chen Jin-Cherng, Liu Kris Sun, Yang Ting-Ju, Hwang Juen-Haur, Chan Yin-Ching, Lee I-Te
出版信息
Nutr Neurosci. 2012 Nov;15(6):252-6. doi: 10.1179/1476830512Y.0000000020.
OBJECTIVE
Our aim was to investigate the effects of Spirulina on BV-2 microglial cell cytotoxicity and inflammatory genes expression.
METHODS
BV-2 microglial cells were treated with lipopolysaccharide (LPS) (1 µg/ml) and various concentrations of Spirulina platensis water extract or its active component (C-phycocyanin (C-PC)) for 24 hours. Cytotoxicity (lactate dehydrogenase (LDH) release) and expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) mRNAs were assayed.
RESULTS
LPS increased LDH production and up-regulated expression of iNOS, COX-2, TNF-α, and IL-6 by BV-2 microglial cells. However, Spirulina platensis water extract and C-PC significantly reduced LPS-induced LDH release, and expression of iNOS, COX-2, TNF-α, and IL-6 mRNAs.
CONCLUSION
Spirulina can reduce the cytotoxicity and inhibit expression of inflammation-related genes of LPS-stimulated BV-2 microglial cells.
目的
我们的目的是研究螺旋藻对BV - 2小胶质细胞毒性和炎症相关基因表达的影响。
方法
用脂多糖（LPS）（1微克/毫升）和不同浓度的钝顶螺旋藻水提取物或其活性成分（藻蓝蛋白（C - PC））处理BV - 2小胶质细胞24小时。检测细胞毒性（乳酸脱氢酶（LDH）释放）以及诱导型一氧化氮合酶（iNOS）、环氧化酶 - 2（COX - 2）、肿瘤坏死因子 -α（TNF -α）和白细胞介素 - 6（IL - 6）mRNA的表达。
结果
LPS增加了BV - 2小胶质细胞的LDH产生，并上调了iNOS、COX - 2、TNF -α和IL - 6的表达。然而，钝顶螺旋藻水提取物和C - PC显著降低了LPS诱导的LDH释放以及iNOS、COX - 2、TNF -α和IL - 6 mRNA的表达。
结论
螺旋藻可降低LPS刺激的BV - 2小胶质细胞的细胞毒性并抑制炎症相关基因的表达。
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