Agricultural Research Council-Viticulture Research Center (CRA-VIT), Conegliano (TV), Italy.
Anal Chim Acta. 2012 Jun 30;732:120-9. doi: 10.1016/j.aca.2011.10.045. Epub 2011 Oct 29.
The anthocyanins of 21 hybrid red varieties produced by crossing V. vinifera, V. riparia, V. labrusca, V. lincecumii and V. rupestris species, the profiles for which have not yet been reported, were studied. Profiles were determined by LC/DAD, and identification of single anthocyanins was confirmed by LC/MS precursor-ion analysis. Anthocyanidin precursors (pelargonidin at m/z 271, dephinidin at m/z 303, cyanidin at m/z 287, petunidin at m/z 317, peonidin at m/z 301, and malvidin at m/z 331) and precursors of monoglucoside compounds allowed 24 different compounds to be identified. Analysis of precursor ions of monoglucoside anthocyanins at low capillary voltage revealed the signals of diglucosides only, providing a very selective method for analysis of diglucoside anthocyanins in grape. According to anthocyanin profile, the samples were subdivided into two groups: one characterized by the substantial presence of diglucoside compounds (particularly Seyve Villard 23-399 and Seyve Villard 23-369) and one by the scarce presence or practical absence of diglucosides (Seibel 10878, Burdin 4077, and Galibert 238-35). Particularly interesting for producing anthocyanin for the natural colorant industry were the varieties Siebel 8357, Bacò 30-12 and Terzi 100-31.
对 21 个杂交红品种的花色苷进行了研究，这些品种是通过杂交葡萄（V. vinifera）、河岸葡萄（V. riparia）、美洲种葡萄（V. labrusca）、毛葡萄（V. lincecumii）和山葡萄（V. rupestris）杂交产生的，这些品种的花色苷图谱尚未报道。采用 LC/DAD 法测定花色苷图谱，通过 LC/MS 前体离子分析对单种花色苷进行了鉴定。花色苷前体（天竺葵色素，m/z 271；矢车菊素，m/z 303；花青素，m/z 287；锦葵色素，m/z 317；芍药色素，m/z 301；和飞燕草色素，m/z 331）和单糖苷化合物的前体允许鉴定出 24 种不同的化合物。在低毛细管电压下分析单糖苷花色苷的前体离子仅揭示了双糖苷的信号，为葡萄中双糖苷花色苷的分析提供了一种非常有选择性的方法。根据花色苷图谱，将样品分为两组：一组以双糖苷化合物的大量存在为特征（特别是 Seyve Villard 23-399 和 Seyve Villard 23-369），另一组以双糖苷的稀少存在或几乎不存在为特征（Seibel 10878、Burdin 4077 和 Galibert 238-35）。对于天然着色剂工业生产花色苷特别有意义的品种是 Siebel 8357、Bacò 30-12 和 Terzi 100-31。
