Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7246-9. doi: 10.1002/anie.201203349. Epub 2012 Jun 11.
A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.
一种新型的翻译后修饰氨基酸，巴豆酰赖氨酸（Kcr），通过进化的吡咯赖氨酸-tRNA/合成酶-tRNA 对在细菌和哺乳动物细胞中被基因整合到蛋白质中。这种能够产生具有均一、定点 Kcr 修饰的组蛋白的能力，对于阐明这种最近发现的翻译后修饰的生物学作用将非常有价值。
