将ε-N-2-羟基异丁酰赖氨酸基因整合到重组组蛋白中。
Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones.
作者信息
Xiao Han, Xuan Weimin, Shao Sida, Liu Tao, Schultz Peter G
机构信息
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
出版信息
ACS Chem Biol. 2015 Jul 17;10(7):1599-603. doi: 10.1021/cb501055h. Epub 2015 Apr 29.
Abstract
Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.
摘要
在此，我们报道了一种正交琥珀抑制型吡咯赖氨酸-tRNA合成酶（PylRS）/tRNACUA(Pyl)对的进化，该对在细菌和哺乳动物细胞中对翻译后修饰的氨基酸ε-N-2-羟基异丁酰赖氨酸（HibK）进行基因编码。HibK是一种新型的组蛋白标记，广泛分布于组蛋白中。将HibK位点特异性地整合到蛋白质中的能力为探究这种新发现的翻译后修饰的生物学功能提供了一种有用的工具。
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