Institute of Organic and Biomolecular Chemistry, Georg-August University of Göttingen, Göttingen, Germany.
Planta Med. 2012 Jun;78(10):1020-3. doi: 10.1055/s-0031-1298622. Epub 2012 Jun 12.
Paeciloside A (1) and eight known compounds, acremoauxin A (2), farinosones A (3) and B (4), 1,5-dideoxy-3-C-methyl-arabitol (5), ergosterol, ergosterol peroxide, cerebroside C, and adenosine, were isolated from cultures of Paecilomyces sp. CAFT156, an endophytic fungus occurring in Enantia chlorantha Oliv (Annonaceae) leaves. The structure of the new compound 1 was elucidated using MS, UV, 1D and 2D NMR experiments, while its absolute configuration was established by subsequent single-crystal X-ray diffraction analysis using copper Kα radiation and invariom nonspherical scattering factors. Paeciloside A (1) and compounds 2, 4, and 5 displayed inhibitory effects on two gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus at a concentration of 40 µg per paper disk and moderate cytotoxicity towards brine shrimp larvae (Artemia salina). This study presents the first report on an endophytic fungus isolated from E. chlorantha Oliv and its natural products.
从内生真菌 Paecilomyces sp. CAFT156 的培养物中分离得到了 paeciloside A (1) 和 8 种已知化合物，分别为 acremoauxin A (2)、farinosones A (3) 和 B (4)、1,5-二脱氧-3-C-甲基阿拉伯糖醇 (5)、麦角甾醇、麦角甾醇过氧化物、脑苷脂 C 和腺苷。新化合物 1 的结构通过 MS、UV、1D 和 2D NMR 实验阐明，其绝对构型通过后续的单晶 X 射线衍射分析使用铜 Kα 辐射和 invariom 非球形散射因子确定。paeciloside A (1)、化合物 2、4 和 5 在每个纸片 40 µg 的浓度下对两种革兰氏阳性菌枯草芽孢杆菌和金黄色葡萄球菌表现出抑制作用，对卤虫幼虫（盐水虾）具有中等细胞毒性。本研究首次报道了从 E. chlorantha Oliv 中分离得到的内生真菌及其天然产物。
