Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, PR China.
Mol Carcinog. 2013 Nov;52(11):871-8. doi: 10.1002/mc.21928. Epub 2012 Jun 12.
Interleukin-17 (IL-17) has been shown to play an important role in the pathogenesis of inflammation and autoimmune disorders, and to be elevated in several types of cancer. The present study analyzed polymorphisms in IL-17 gene and their impact on the pathogenesis of bladder cancer. The TaqMan® SNP Genotyping Assay was used to genotype the SNP rs2275913 of IL-17A and SNP rs763780 of IL-17F in 301 bladder cancer patients and 446 ethnicity-matched healthy controls. The frequencies of AA genotype and A allele of rs2275913, as well as TT genotype and T allele of rs763780 in the bladder cancer patients were significantly higher than that of controls, indicating that both of these two SNPs were associated with bladder cancer (P = 0.003, OR = 1.37, 95% CI = 1.12-1.69 for allele A of rs2275913, and P = 0.018, OR = 1.46, 95% CI = 1.07-2.00 for allele T of rs763780, respectively). Results of stratified analysis revealed that rs2275913 was associated with male, non-smokers, and invasion of bladder cancer, while rs763780 was associated with invasion of bladder cancer. Our results suggested that the SNP rs2275913 of IL-17A and SNP rs763780 of IL-17F were associated with the development, as well as gender, smoking status and tumor stage of bladder cancer.
白细胞介素-17（IL-17）在炎症和自身免疫性疾病的发病机制中起着重要作用，并在几种类型的癌症中升高。本研究分析了白细胞介素-17 基因的多态性及其对膀胱癌发病机制的影响。采用 TaqMan® SNP 基因分型检测试剂盒对 301 例膀胱癌患者和 446 名匹配的健康对照者的 IL-17A 基因 rs2275913 多态性和 IL-17F 基因 rs763780 多态性进行基因分型。膀胱癌患者 rs2275913 的 AA 基因型和 A 等位基因以及 rs763780 的 TT 基因型和 T 等位基因的频率明显高于对照组，表明这两个 SNP 均与膀胱癌相关（P=0.003，OR=1.37，95%CI=1.12-1.69，等位基因 A；P=0.018，OR=1.46，95%CI=1.07-2.00，等位基因 T）。分层分析结果表明，rs2275913 与男性、非吸烟者和膀胱癌浸润有关，而 rs763780 与膀胱癌浸润有关。我们的结果表明，IL-17A 基因 rs2275913 多态性和 IL-17F 基因 rs763780 多态性与膀胱癌的发生以及性别、吸烟状态和肿瘤分期有关。
