School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Anal Bioanal Chem. 2012 Aug;404(2):585-92. doi: 10.1007/s00216-012-6123-3. Epub 2012 Jun 14.
We report herein a novel chemiluminescence (CL) phenomenon triggered by light irradiation when a fluorescent dye, for example hematoporphyrin, fluorescein, eosin, or methylene blue is present in the luminol solution. A possible mechanism is proposed for the photoinduced chemiluminescence (PICL) reaction. Compared with reported methods for CL triggering, for example flow-injection, static reagent injection, and the electrochemical technique, the proposed in-situ PICL method presented has three advantages. First, the method is more selective, because the PICL signal of the target fluorescent dyes is initiated by excitation at a selective wavelength only. Second, the space and time resolution of the PICL method are better. Last, and most important, compared with injecting a reagent or inserting a electrode into the CL system to initiate the CL reaction, with the in-situ PICL method there is no physical interference with the target detecting system. All these advantages of the PICL method indicate it has many potential applications in the analytical sciences. The proposed method was applied to analysis of urine containing adrenaline. The linear range for adrenaline is 2.0 × 10(-10)-1.0 × 10(-7) g mL(-1) and the detection limit is 6.0 × 10(-11) g mL(-1).
我们在此报告一种新型的化学发光（CL）现象，当荧光染料（例如血卟啉、荧光素、曙红或亚甲蓝）存在于鲁米诺溶液中时，通过光照会引发这种现象。我们提出了一种可能的光致化学发光（PICL）反应机制。与已报道的 CL 触发方法（例如流动注射、静态试剂注入和电化学技术）相比，所提出的原位 PICL 方法具有三个优点。首先，该方法更具选择性，因为只有在特定波长的激发下，目标荧光染料的 PICL 信号才会被触发。其次，PICL 方法的空间和时间分辨率更好。最后，也是最重要的一点，与将试剂注入或插入 CL 系统以引发 CL 反应相比，原位 PICL 方法不会对目标检测系统造成物理干扰。PICL 方法的所有这些优点表明，它在分析科学中有许多潜在的应用。该方法被应用于分析尿液中的肾上腺素。肾上腺素的线性范围为 2.0×10(-10)-1.0×10(-7) g mL(-1)，检测限为 6.0×10(-11) g mL(-1)。
