环磷酸腺苷作为多顺反子转录单位中极性的调节剂。
Cyclic AMP as a modulator of polarity in polycistronic transcriptional units.
作者信息
Ullmann A, Joseph E, Danchin A
出版信息
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3194-7. doi: 10.1073/pnas.76.7.3194.
Abstract
The degree of natural polarity in the lactose and galactose operons of Escherichia coli is affected by adenosine 3',5'-cyclic monophosphate (cAMP). This effect, mediated by the cAMP receptor protein, is exerted at sites distinct from the promoter. Experiments performed with a mutant bearing a thermosensitive rho factor activity indicate that cAMP relieves polarity by interfering with transcription termination. Conflicting results in the literature concerning the role of cAMP receptor protein and cAMP in galactose operon expression can be reconciled by the findings that cAMP stimulates the expression of operator distal genes without significantly affecting the proximal genes. Therefore, it appears necessary to reevaluate the classification of the galactose operon as exhibiting cAMP-mediated catabolite repression at the level of transcription initiation.
摘要
大肠杆菌乳糖和半乳糖操纵子中的自然极性程度受3',5'-环磷酸腺苷（cAMP）影响。这种由cAMP受体蛋白介导的效应作用于与启动子不同的位点。用带有热敏性rho因子活性的突变体进行的实验表明，cAMP通过干扰转录终止来缓解极性。文献中关于cAMP受体蛋白和cAMP在半乳糖操纵子表达中的作用存在相互矛盾的结果，而cAMP刺激操纵基因远端基因的表达但不显著影响近端基因这一发现可以解释这些矛盾。因此，似乎有必要重新评估将半乳糖操纵子归类为在转录起始水平表现出cAMP介导的分解代谢物阻遏的分类。
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Bearing of some recent results on the mechanisms of polarity and messenger RNA stability.一些近期结果对极性和信使核糖核酸稳定性机制的影响
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In vitro transcription of the gal operon requires cyclic adenosine monophosphate and cyclic adenosine monophosphate receptor protein.半乳糖操纵子的体外转录需要环磷酸腺苷和环磷酸腺苷受体蛋白。
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