体外基因表达对DNA回旋酶抑制剂的差异敏感性。
Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.
作者信息
Yang H L, Heller K, Gellert M, Zubay G
出版信息
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3304-8. doi: 10.1073/pnas.76.7.3304.
Abstract
We have used the antibiotics coumermycin A1, novobiocin, and oxolinic acid, which are specific inhibitors of DNA gyrase, to study the coupled transcription and translation of several bacterial and plasmid genes in a DNA-directed cell-free system. The expression of different genes is reduced to different extents by inhibition of DNA gyrase activity. Among the genes tested, the lac operon, an rRNA gene, and the colicin gene of colicin E1 plasmid were found to be most sensitive, while the trp operon and some other genes in colicin E1 plasmid were relatively unaffected by the inhibitors. These results, together with earlier work on the transcription of circular DNA templates, indicate that DNA supercoiling can significantly enhance transcription from certain promoters.
摘要
我们使用了香豆霉素A1、新生霉素和恶喹酸这几种抗生素，它们是DNA回旋酶的特异性抑制剂，用于研究在DNA指导的无细胞系统中几种细菌和质粒基因的转录与翻译偶联过程。通过抑制DNA回旋酶活性，不同基因的表达受到不同程度的降低。在测试的基因中，发现乳糖操纵子、一个rRNA基因以及大肠杆菌素E1质粒的大肠杆菌素基因最为敏感，而色氨酸操纵子和大肠杆菌素E1质粒中的其他一些基因相对不受抑制剂的影响。这些结果，连同早期关于环状DNA模板转录的研究工作，表明DNA超螺旋能够显著增强某些启动子的转录。
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