来自人类免疫缺陷病毒1型的核糖体移码信号在大肠杆菌中的功能。
The function of a ribosomal frameshifting signal from human immunodeficiency virus-1 in Escherichia coli.
作者信息
Yelverton E, Lindsley D, Yamauchi P, Gallant J A
机构信息
University of Washington, Department of Genetics, Seattle 98195.
出版信息
Mol Microbiol. 1994 Jan;11(2):303-13. doi: 10.1111/j.1365-2958.1994.tb00310.x.
A 15-17 nucleotide sequence from the gag-pol ribosome frameshift site of HIV-1 directs analogous ribosomal frameshifting in Escherichia coli. Limitation for leucine, which is encoded precisely at the frameshift site, dramatically increased the frequency of leftward frameshifting. Limitation for phenylalanine or arginine, which are encoded just before and just after the frameshift, did not significantly affect frameshifting. Protein sequence analysis demonstrated the occurrence of two closely related frameshift mechanisms. In the first, ribosomes appear to bind leucyl-tRNA at the frameshift site and then slip leftward. This is the 'simultaneous slippage' mechanism. In the second, ribosomes appear to slip before binding aminoacyl-tRNA, and then bind phenylalanyl-tRNA, which is encoded in the left-shifted reading frame. This mechanism is identical to the 'overlapping reading' we have demonstrated at other bacterial frameshift sites. The HIV-1 sequence is prone to frame-shifting by both mechanisms in E. coli.
来自HIV-1的gag-pol核糖体移码位点的一段15 - 17个核苷酸的序列在大肠杆菌中引导类似的核糖体移码。对亮氨酸的限制（亮氨酸恰好在移码位点编码）显著增加了向左移码的频率。对苯丙氨酸或精氨酸的限制（它们分别在移码之前和之后编码）并没有显著影响移码。蛋白质序列分析表明存在两种密切相关的移码机制。第一种机制中，核糖体似乎在移码位点结合亮氨酰 - tRNA，然后向左滑动。这就是“同时滑动”机制。第二种机制中，核糖体似乎在结合氨酰 - tRNA之前就滑动，然后结合在向左移动的阅读框中编码的苯丙氨酰 - tRNA。这种机制与我们在其他细菌移码位点所证明的“重叠阅读”相同。HIV-1序列在大肠杆菌中易于通过这两种机制发生移码。
Zotero 插件