Zitomer R S, Montgomery D L, Nichols D L, Hall B D
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627-31. doi: 10.1073/pnas.76.8.3627.
DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.
来自克隆的酵母同工酶-1-细胞色素c（cycl）基因的DNA被用于杂交试验，以测量cycl RNA的合成水平和速率。去阻遏细胞合成cycl RNA的速率是葡萄糖阻遏细胞的6倍。向去阻遏培养物中添加葡萄糖后，cycl基因的转录在2.5分钟内被抑制。在阻遏和去阻遏条件下以及阻遏过程中，可杂交的cycl RNA的半衰期被确定为12 - 13.5分钟。结果表明，cycl基因的表达受到转录调控。
