Medicinal Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226015, India.
Phytochem Anal. 2013 Jan-Feb;24(1):87-92. doi: 10.1002/pca.2387. Epub 2012 Jul 11.
Solanum species are important ingredients of many traditional Indian medicines and thus the quality control of their herbal formulations is of paramount concern.
To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate the quality of Solanum species and their herbal formulations.
A rapid, simple, sensitive, robust and reproducible HPLC method was developed for the determination of three steroidal glycosides (SG); indioside D, solamargine and α-solanine in eight species of the genus Solanum. The analytes were separated on a monolithic performance RP-18e column (100 mm × 4.6 mm i.d.) using a gradient elution of acetonitile-water containing 0.1% trifluoroacetic acid (TFA) as the mobile phase with a flow rate 0.4 mL/min and UV detection at λ 210 nm.
The method was linear over the range 3-15 µg/mL (r > 9994). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at the three concentrations were higher than 98.8% with RSD < 2% for the targets.
The established method is simple and can be used as a tool for quality control of plant material or herbal formulation containing SG.
茄属植物是许多印度传统药物的重要成分，因此其草药配方的质量控制至关重要。
建立一种简单有效的高效液相色谱（HPLC）方法，以评估茄属植物及其草药配方的质量。
开发了一种快速、简单、灵敏、稳健且重现性好的 HPLC 方法，用于测定 8 种茄属植物中的三种甾体糖苷（SG）；靛苷 D、茄碱和α-龙葵碱。分析物在整体性能 RP-18e 柱（100mm×4.6mm id）上分离，使用含 0.1%三氟乙酸（TFA）的乙腈-水作为流动相进行梯度洗脱，流速为 0.4mL/min，UV 检测波长为 λ 210nm。
该方法在 3-15μg/mL 范围内呈线性（r>9994）。准确度、精密度和重复性均在要求范围内。在三个浓度下测量的平均回收率均高于 98.8%，目标物的 RSD<2%。
所建立的方法简单，可用于含有 SG 的植物材料或草药配方的质量控制。
