评价鬼针草叶乙醇提取物的平喘活性。
Evaluation of antiasthmatic activity of ethanolic extract of Elephantopus scaber L. leaves.
机构信息
Department of Pharmacognosy and Phytochemistry, Dr. H. S Gour Central University, Sagar, Madhya Pradesh, India.
出版信息
Indian J Pharmacol. 2012 May;44(3):398-401. doi: 10.4103/0253-7613.96347.
AIMS
The aim of study was to evaluate the scientific basis for the traditional use of Elephantopus scaber leaves.
MATERIALS AND METHODS
In the present study, ethanol extract of Elephantopus scaber leaves was evaluated for preliminary phytochemical screening and antiasthmatic activity using histamine and acetylcholine-induced bronchospasm, mast cell degranulation and histamine induced constriction on isolated guinea pig tracheal chain at different dose levels. Student's t-Test and Dunett's test were used for statistical analysis.
RESULTS
The result of present investigation showed that the ethanolic extract of E. scaber significantly (P<0.001) decreased the bronchospasm induced by histamine, acetylcholine and protected mast cell degranulation as compared to control groups. It also decreased the histamine induce constriction on isolated guinea pig trachea in dose-dependent manner. Phytochemical studies revealed the presence of steroids, saponin, flavonoids, and phenolic compounds in the extract.
CONCLUSIONS
The present study concludes that the antiasthmatic activity of ethanolic extract of E. scaber leaves may be due to the presence of flavonoids or steroids. Antiasthmatic action of the E. scaber could be due to its antihistaminic, anticholinergic and mast-cell-stabilizing property.
目的
本研究旨在评估地胆草叶传统用途的科学基础。
材料和方法
在本研究中，我们评估了地胆草叶乙醇提取物的初步植物化学筛选和抗哮喘活性，使用组胺和乙酰胆碱诱导的支气管痉挛、肥大细胞脱颗粒以及在不同剂量水平下对分离的豚鼠气管链的组胺诱导收缩来进行研究。采用学生 t 检验和 Dunnett 检验进行统计学分析。
结果
目前的研究结果表明，与对照组相比，地胆草叶的乙醇提取物显著（P<0.001）降低了由组胺、乙酰胆碱引起的支气管痉挛，并保护了肥大细胞脱颗粒。它还以剂量依赖的方式降低了组胺诱导的分离豚鼠气管收缩。植物化学研究表明提取物中存在甾体、皂苷、类黄酮和酚类化合物。
结论
本研究得出结论，地胆草叶乙醇提取物的抗哮喘活性可能是由于存在黄酮类化合物或甾体类化合物。地胆草的抗哮喘作用可能是由于其具有抗组胺、抗胆碱能和稳定肥大细胞的特性。
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