Fittipaldi Raffaella, Caretti Giuseppina
Department of Biomolecular Sciences and Biotechnology, University of Milano, Via Celoria 26, 20133 Milan, Italy.
Comp Funct Genomics. 2012;2012:979168. doi: 10.1155/2012/979168. Epub 2012 Jun 3.
Recent advances in high-throughput technologies have transformed methodologies employed to study cell-specific epigenomes and the approaches to investigate complex cellular phenotypes. Application of next-generation sequencing technology in the skeletal muscle differentiation field is rapidly extending our knowledge on how chromatin modifications, transcription factors and chromatin regulators orchestrate gene expression pathways guiding myogenesis. Here, we review recent biological insights gained by the application of next-generation sequencing techniques to decode the epigenetic profile and gene regulatory networks underlying skeletal muscle differentiation.
高通量技术的最新进展改变了用于研究细胞特异性表观基因组的方法以及研究复杂细胞表型的途径。下一代测序技术在骨骼肌分化领域的应用正在迅速拓展我们对于染色质修饰、转录因子和染色质调节因子如何协调指导肌生成的基因表达途径的认识。在此，我们综述了通过应用下一代测序技术来解码骨骼肌分化背后的表观遗传图谱和基因调控网络所获得的最新生物学见解。
