检测伊斯坦布尔血库献血者血液中的抗利什曼原虫抗体。

Detection of antileishmanial antibodies in blood sampled from blood bank donors in Istanbul.

机构信息

Yildiz Technical University, Bioengineering Department, Istanbul, Turkey.

出版信息

Future Microbiol. 2012 Jun;7(6):773-9. doi: 10.2217/fmb.12.46.

DOI:10.2217/fmb.12.46

PMID:22702529

Abstract

AIMS

According to the WHO, only 5-20% of the total cases of leishmaniasis are symptomatic leishmaniasis; the other cases are identified as asymptomatic leishmaniasis. In recent studies, it has been demonstrated that donor blood plays an important role in the epidemiology of asymptomatic leishmaniasis. However, the number of the studies on this subject is still insufficient. Additionally, donor blood samples obtained from Istanbul, which is the biggest metropolitan area in Turkey, have not been investigated with regard to Leishmania. Moreover, there is no information about the sensitivity of noninvasive serological methods that are used in the detection of leishmaniasis donor blood samples. Accordingly, this study aimed to investigate the presence of antileishmanial antibodies in blood samples obtained from blood bank donors in Istanbul, by using different serologic methods, and to determine the most sensitive detection method.

MATERIALS & METHODS: Blood samples were taken from 188 healthy blood bank donors to the Capa Turkish Red Crescent Blood Bank (Istanbul, Turkey), and the presence of antileishmanial antibodies was measured by indirect immunofluorescent antibody test (IFAT), ELISA, immunochromatographic dipstick rapid test, and western blot (WB).

RESULTS

Antileishmanial antibodies were determined in 12 out of 188 samples by IFAT (6.4%), and six out of these 12 donors were found to be positive at diagnostic titer 1:128 (3.2%). One hundred and eighty eight samples were investigated by ELISA and one (0.5%) of them gave a positive result. None of 188 samples provided a positive result by immunochromatographic test. WB applied to the 12 seroreactive donors showed that three out of 12 donors were positive.

CONCLUSION

In this study, the presence of antileishmanial antibodies in blood samples of blood bank donors from Istanbul has been demonstrated by using feasible and low-cost serological methods. Additionally, in comparison with other simple and low-cost detection methods, WB was used for confirmation. IFAT has a higher sensitivity and therefore may be preferred as a prescreening method in endemic or nonendemic areas.

摘要

目的

根据世界卫生组织（WHO）的数据，利什曼病总病例中只有 5-20%为有症状利什曼病；其他病例则被认定为无症状利什曼病。最近的研究表明，供体血液在无症状利什曼病的流行病学中起着重要作用。然而，关于这一主题的研究数量仍然不足。此外，尚未对来自土耳其最大的都市区伊斯坦布尔的供体血液样本进行利什曼研究。此外，关于用于检测供体血液样本中利什曼病的非侵入性血清学方法的敏感性也没有相关信息。因此，本研究旨在通过使用不同的血清学方法检测来自伊斯坦布尔血液银行供体的血液样本中是否存在抗利什曼原虫抗体，并确定最敏感的检测方法。

材料和方法

从卡帕土耳其红新月会血液银行（土耳其伊斯坦布尔）的 188 名健康献血者中采集血液样本，通过间接免疫荧光抗体试验（IFA）、酶联免疫吸附试验（ELISA）、免疫层析快速检测棒和免疫印迹（WB）来检测抗利什曼原虫抗体。

结果

通过 IFA 在 188 个样本中确定了 12 个样本中的抗利什曼原虫抗体（6.4%），其中 12 个供体中有 6 个在诊断滴度为 1:128（3.2%）时呈阳性。用 ELISA 检测了 188 个样本，其中 1 个（0.5%）呈阳性。188 个样本均未通过免疫层析检测呈阳性。应用于 12 个血清反应性供体的 WB 显示，其中 3 个供体呈阳性。