Yildiz Technical University, Bioengineering Department, Istanbul, Turkey.
Acta Trop. 2013 Oct;128(1):54-60. doi: 10.1016/j.actatropica.2013.06.009. Epub 2013 Jun 24.
In recent years, the role of donor blood has taken an important place in epidemiology of Leishmaniasis. According to the WHO, the numbers of patients considered as symptomatic are only 5-20% of individuals with asymptomatic leishmaniasis. In this study for detection of Leishmania infection in donor blood samples, 343 samples from the Capa Red Crescent Blood Center were obtained and primarily analyzed by microscopic and serological methods. Subsequently, the traditional culture (NNN), Immuno-chromatographic test (ICT) and Polymerase Chain Reaction (PCR) methods were applied to 21 samples which of them were found positive with at least one method. Buffy coat (BC) samples from 343 blood donors were analyzed: 15 (4.3%) were positive by a microculture method (MCM); and 4 (1.1%) by smear. The sera of these 343 samples included 9 (2.6%) determined positive by ELISA and 7 (2%) positive by IFAT. Thus, 21 of (6.1%) the 343 subjects studied by smear, MCM, IFAT and ELISA techniques were identified as positive for leishmaniasis at least one of the techniques and the sensitivity assessed. According to our data, the sensitivity of the methods are identified as MCM (71%), smear (19%), IFAT (33%), ELISA (42%), NNN (4%), PCR (14%) and ICT (4%). Thus, with this study for the first time, the sensitivity of a MCM was examined in blood donors by comparing MCM with the methods used in the diagnosis of leishmaniasis. As a result, MCM was found the most sensitive method for detection of Leishmania parasites in samples obtained from a blood bank. In addition, the presence of Leishmania parasites was detected in donor bloods in Istanbul, a non-endemic region of Turkey, and these results is a vital importance for the health of blood recipients.
近年来，供体血液在利什曼病的流行病学中扮演了重要角色。根据世界卫生组织的说法，被认为有症状的患者人数仅占无症状利什曼病患者的 5-20%。在这项研究中，为了检测供体血液样本中的利什曼原虫感染，从卡帕红新月血液中心获得了 343 份样本，并首先通过显微镜和血清学方法进行了分析。随后，对 21 份样本应用了传统培养（NNN）、免疫层析检测（ICT）和聚合酶链反应（PCR）方法，其中至少有一种方法呈阳性。对 343 名献血者的血涂片样本进行了分析：15 份（4.3%）通过微培养法（MCM）呈阳性；4 份（1.1%）通过涂片呈阳性。这些 343 份血清样本中，9 份（2.6%）通过 ELISA 确定为阳性，7 份（2%）通过 IFAT 确定为阳性。因此，通过涂片、MCM、IFAT 和 ELISA 技术对 343 名研究对象中的 21 名进行了检测，这些技术至少有一种检测方法确定为利什曼病阳性，评估了其敏感性。根据我们的数据，这些方法的敏感性被确定为 MCM（71%）、涂片（19%）、IFAT（33%）、ELISA（42%）、NNN（4%）、PCR（14%）和 ICT（4%）。因此，通过这项研究，我们首次通过比较 MCM 与利什曼病诊断中使用的方法，在献血者中检测了 MCM 的敏感性。结果表明，MCM 是从血库中获得的样本中检测利什曼寄生虫最敏感的方法。此外，在土耳其非利什曼病流行地区伊斯坦布尔的献血者血液中也检测到了利什曼寄生虫，这些结果对血液接受者的健康至关重要。
