Bendale Yogesh, Bendale Vineeta, Paul Saili, Bhattacharyya Soumya Sundar
Research and Development Section, Cell and Molecular Biology Department, Rasayani Biologics Private Limited, Pune 411030, India.
Zhong Xi Yi Jie He Xue Bao. 2012 Jun;10(6):681-9. doi: 10.3736/jcim20120613.
In the present study, the anticancer potential of platinum nanoparticles Bioplatin is explored and the mode of interactions of Bioplatin with calf thymus DNA and honey was analyzed.
Bioplatin was synthesized with the help of green nanotechnology and characterized by particle size, zeta potential and surface morphology. The interaction of Bioplatin with DNA and honey was also checked with the help of circular dichroism spectroscopy and Fourier-transform infrared spectroscopy, respectively. The anticancer potential of Bioplatin was evaluated on peripheral blood mononuclear cells and A375 cells in vitro by analyzing results of MTT (3-(4,5)-dimethyl-thiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide), fluorescence microscopic studies and DNA fragmentation assay.
Bioplatin exhibited a small particle size of 137.5 nm and a surface charge of -35.8 mV. Bioplatin interacted with DNA and brought in effective changes in structure and conformation of DNA, and formed a new complex that increased its stability of DNA intercalated with the base pair of DNA. In vitro studies demonstrated that Bioplatin arrested cell proliferation, and induced chromatin condensation and internucleosomal DNA fragmentation.
Bioplatin induces apoptosis in cancer cells and may have some beneficial effect against human carcinoma. It interacts with DNA, brings stabilization to DNA, and thus prevents the replication of DNA.
在本研究中，探索了铂纳米颗粒生物铂的抗癌潜力，并分析了生物铂与小牛胸腺DNA和蜂蜜的相互作用模式。
借助绿色纳米技术合成生物铂，并通过粒径、zeta电位和表面形态进行表征。分别借助圆二色光谱和傅里叶变换红外光谱检测生物铂与DNA和蜂蜜的相互作用。通过分析MTT（3-(4,5)-二甲基噻唑-(-z-y1)-3,5-二苯基四氮唑溴盐）结果、荧光显微镜研究和DNA片段化分析，在体外评估生物铂对人外周血单个核细胞和A375细胞的抗癌潜力。
生物铂呈现出137.5 nm的小粒径和-35.8 mV的表面电荷。生物铂与DNA相互作用，使DNA的结构和构象发生有效变化，并形成一种新的复合物，增强了与DNA碱基对插入的DNA的稳定性。体外研究表明，生物铂可阻止细胞增殖，并诱导染色质浓缩和核小体间DNA片段化。
生物铂可诱导癌细胞凋亡，可能对人类癌症有一定的有益作用。它与DNA相互作用，使DNA稳定，从而阻止DNA复制。
