Banerjee Kaushik, Das Satyajit, Choudhury Pritha, Ghosh Sarbari, Baral Rathindranath, Choudhuri Soumitra Kumar
Department of In Vitro Carcinogenesis and Cellular Chemotherapy, Chittaranjan National Cancer Institute, Kolkata, India.
Chemotherapy. 2017;62(5):279-289. doi: 10.1159/000453446. Epub 2017 May 11.
Development of novel strategies to kill cancer by sparing normal cells is of utmost importance. Apart from their known antimicrobial activity, only limited information has been recorded regarding the antitumor potential of biocompatible silver oxide nanoparticles (AgONPs). There is a need to evaluate the anticancer potential of biocompatible AgONPs in vitro.
A new approach of utilizing the leaf extract of Excoecaria agallocha was used to synthesize AgONPs. This was then characterized by ultraviolet-visible spectrophotometry, nanoparticle-tracking analysis, and ζ-potential analysis. Cytotoxicity and apoptotic potential were evaluated with an MTT assay and an annexin V-binding assay against the murine melanoma (B16F10), murine colon cancer (CT26), murine lung adenocarcinoma (3LL), and murine Ehrlich ascites carcinoma (EAC) cell lines. Cellular localization of AgONPs was evaluated on fluorescence microscopy.
UV peaks at 270 and 330 nm indicated the formation of nanoparticles (NPs) and the NP-tracking analyzer revealed them to have a size of 228 nm. AgONPs exerted initial cytotoxicity, specifically against all the experimental malignant cells by sparing the normal cell lines. Moreover, AgONPs exert apoptosis equally on all the malignant cells in vitro and ex vivo. This cytotoxicity possibly occurs via the nuclear translocation of AgONPs as analyzed in B16F10 cells.
AgONPs utilizing natural sources would be a new medicinal approach against a broad spectrum of malignancy.
开发能够在不损伤正常细胞的情况下杀死癌细胞的新策略至关重要。除了已知的抗菌活性外，关于生物相容性氧化银纳米颗粒（AgONPs）的抗肿瘤潜力，仅有有限的信息被记录。有必要在体外评估生物相容性AgONPs的抗癌潜力。
采用一种利用海漆叶提取物合成AgONPs的新方法。然后通过紫外可见分光光度法、纳米颗粒跟踪分析和ζ电位分析对其进行表征。采用MTT法和膜联蛋白V结合试验，针对小鼠黑色素瘤（B16F10）、小鼠结肠癌（CT26）、小鼠肺腺癌（3LL）和小鼠艾氏腹水癌（EAC）细胞系评估细胞毒性和凋亡潜力。通过荧光显微镜评估AgONPs的细胞定位。
270和330nm处的紫外峰表明纳米颗粒（NPs）的形成，纳米颗粒跟踪分析仪显示其尺寸为228nm。AgONPs表现出初始细胞毒性，通过不损伤正常细胞系，特异性地针对所有实验性恶性细胞。此外，AgONPs在体外和体内对所有恶性细胞均同等地诱导凋亡。如在B16F10细胞中分析的那样，这种细胞毒性可能通过AgONPs的核转位发生。
利用天然来源的AgONPs将是一种针对广泛恶性肿瘤的新的药物治疗方法。
