Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Food Chem Toxicol. 2012 Sep;50(9):3086-93. doi: 10.1016/j.fct.2012.05.055. Epub 2012 Jun 13.
Mulberry extracts are antidiabetic and antihyperlipidemic, as well as preventive of cardiovascular disease. The current study investigates the protective mechanisms of mulberry water extracts (MWEs) in carbon tetrachloride (CCl(4))-induced hepatic injury. Oral administration of MWEs significantly reduced the lipid peroxidation triggered by CCl(4), as shown by the reduced production of thiobarituric acid-reactive substance (TBARS). The levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were also reduced via cotreatment with MWEs compared with CCl(4) treatment alone. Cotreatment with MWE evidently reduced CCl(4)-induced liver weight and inhibited lipid deposition and fibrogenesis. In a similar manner, cotreatment with silymarin, a well-known liver protective agent, also reversed the CCl(4)-induced effects, such as reduced TBARS formation, decreased serum AST, ALT, and ALP levels, blocked lipid accumulation, and liver fibrosis. Furthermore, MWEs attenuated the proinflammatory genes such as cyclooxygenase 2, nuclear factor kappa B, and inducible nitric oxide synthase expression. The current findings suggest that MWEs such as silymarin exhibit protective and curative effects against CCl(4)-induced liver damage and fibrosis via decreased lipid peroxidation and inhibited proinflammatory gene expression.
桑椹提取物具有降血糖、降血脂、预防心血管疾病的作用。本研究探讨了桑椹水提取物（MWEs）对四氯化碳（CCl4）诱导肝损伤的保护机制。MWEs 的口服给药可显著降低 CCl4 引发的脂质过氧化，表现为硫代巴比妥酸反应物质（TBARS）生成减少。与单独用 CCl4 处理相比，用 MWEs 共同处理还降低了血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）的水平。MWEs 还明显减轻 CCl4 引起的肝重，并抑制脂质沉积和纤维形成。以同样的方式，用众所周知的肝脏保护剂水飞蓟素共同处理也逆转了 CCl4 引起的作用，如 TBARS 形成减少、血清 AST、ALT 和 ALP 水平降低、阻止脂质积累和肝纤维化。此外，MWEs 还减弱了环氧合酶 2、核因子 κB 和诱导型一氧化氮合酶等促炎基因的表达。这些研究结果表明，MWEs 类如水飞蓟素通过降低脂质过氧化和抑制促炎基因表达，对 CCl4 诱导的肝损伤和纤维化具有保护和治疗作用。
