Hacettepe University, Faculty of Medicine, Department of Medical Microbiology, 06100 Ankara, Turkey.
J Infect. 2012 Oct;65(4):343-9. doi: 10.1016/j.jinf.2012.05.010. Epub 2012 Jun 13.
Flavi- and Phleboviruses associated with central nervous system (CNS) infections including West Nile Virus (WNV), Tick-borne Encephalitis Virus (TBEV) and Toscana Virus (TOSV) cause significant morbidity and mortality in humans. In this study, the impact of these agents have been investigated in CNS infections at referral hospitals in two provinces in Turkey, where circulation of these viruses have previously been recognized.
In the study, 258 samples from 126 individuals from Ankara and 113 samples from 108 individuals from Izmir provinces collected in 2010 were included. Viral RNAs were investigated by multiple genus and strain specific primers. Commercial serological assays were employed in screening and reactive results were evaluated with additional assays and by plaque reduction neutralization assay.
Two cases of WNV CNS infections, 14 cases of TOSV infections and one TBEV-exposed individual were identified via serological testing. WNV infections in 61 and 56-year old individuals from Ankara presented with fever and encephalitis without skin rash and residual neurologic damage. TOSV-associated cases from both provinces mainly displayed signs of meningitis. TOSV exposure was documented for the first time from Izmir.
WNV, TBEV and TOSV infections must be considered in cases of meningoencephalitis of unknown etiology in Turkey.
与中枢神经系统（CNS）感染相关的黄病毒属和白纹伊蚊病毒属，包括西尼罗河病毒（WNV）、蜱传脑炎病毒（TBEV）和托斯卡纳病毒（TOSV），在人类中引起重大发病率和死亡率。在这项研究中，研究了这些病原体在土耳其两省转诊医院的 CNS 感染中的影响，此前在这些地区已经发现了这些病毒的循环。
在这项研究中，包括 2010 年收集的来自安卡拉的 126 名个体的 258 份样本和来自伊兹密尔的 108 名个体的 113 份样本。使用多种属和株特异性引物检测病毒 RNA。采用商业血清学检测方法进行筛选，并用其他检测方法和噬斑减少中和试验评估反应性结果。
通过血清学检测鉴定出两例 WNV CNS 感染、14 例 TOSV 感染和 1 例 TBEV 暴露个体。安卡拉 61 岁和 56 岁的 WNV 感染患者表现为发热性脑炎，无皮疹和残留的神经损伤。两省的 TOSV 相关病例主要表现为脑膜炎症状。首次从伊兹密尔记录到 TOSV 暴露。
在土耳其，对于病因不明的脑膜炎病例，必须考虑WNV、TBEV 和 TOSV 感染的可能性。
