Alkan Cigdem, Alwassouf Sulaf, Piorkowski Géraldine, Bichaud Laurence, Tezcan Seda, Dincer Ender, Ergunay Koray, Ozbel Yusuf, Alten Bulent, de Lamballerie Xavier, Charrel Rémi N
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranee Infection, APHM Public Hospitals of Marseille, Marseille, France.
Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France.
J Virol. 2015 Apr;89(8):4080-91. doi: 10.1128/JVI.03027-14. Epub 2015 Feb 4.
A new phlebovirus, Adana virus, was isolated from a pool of Phlebotomus spp. (Diptera; Psychodidae) in the province of Adana, in the Mediterranean region of Turkey. Genetic analysis based on complete coding of genomic sequences indicated that Adana virus belongs to the Salehabad virus species of the genus Phlebovirus in the family Bunyaviridae. Adana virus is the third virus of the Salehabad virus species for which the complete sequence has been determined. To understand the epidemiology of Adana virus, a seroprevalence study using microneutralization assay was performed to detect the presence of specific antibodies in human and domestic animal sera collected in Adana as well as Mersin province, located 147 km west of Adana. The results demonstrate that the virus is present in both provinces. High seroprevalence rates in goats, sheep, and dogs support intensive exposure to Adana virus in the region, which has not been previously reported for any virus included in the Salehabad serocomplex; however, low seroprevalence rates in humans suggest that Adana virus is not likely to constitute an important public health problem in exposed human populations, but this deserves further studies.
Until recently, in the genus Phlebovirus, the Salehabad virus species consisted of two viruses: Salehabad virus, isolated from sand flies in Iran, and Arbia virus, isolated from sand flies in Italy. Here we present the isolation and complete genome characterization of the Adana virus, which we propose to be included in the Salehabad virus species. To our knowledge, this is the first report of the isolation and complete genome characterization, from sand flies in Turkey, of a Salehabad virus-related phlebovirus with supporting seropositivity in the Mediterranean, Aegean, and Central Anatolia regions, where phleboviruses have been circulating and causing outbreaks. Salehabad species viruses have generally been considered to be a group of viruses with little medical or veterinary interest. This view deserves to be revisited according to our results, which indicate a high animal infection rate of Adana virus and recent evidence of human infection with Adria virus in Greece.
一种新的白蛉病毒——阿达纳病毒，从土耳其地中海地区阿达纳省的一组白蛉（双翅目；蛾蠓科）中分离出来。基于基因组序列完整编码的遗传分析表明，阿达纳病毒属于布尼亚病毒科白蛉病毒属的萨勒哈巴德病毒种。阿达纳病毒是已确定完整序列的萨勒哈巴德病毒种的第三种病毒。为了解阿达纳病毒的流行病学情况，采用微量中和试验进行了血清流行率研究，以检测在阿达纳以及位于阿达纳以西147公里处的梅尔辛省采集的人类和家畜血清中特异性抗体的存在情况。结果表明该病毒在这两个省份均有存在。山羊、绵羊和狗的血清流行率较高，这表明该地区存在大量阿达纳病毒暴露情况，此前萨勒哈巴德血清复合体中的任何病毒都未出现过这种情况；然而，人类的血清流行率较低，这表明阿达纳病毒不太可能在暴露的人群中构成重大公共卫生问题，但这值得进一步研究。
直到最近，在白蛉病毒属中，萨勒哈巴德病毒种仅包含两种病毒：从伊朗沙蝇中分离出的萨勒哈巴德病毒，以及从意大利沙蝇中分离出的阿尔比亚病毒。在此，我们展示了阿达纳病毒的分离及完整基因组特征，我们建议将其纳入萨勒哈巴德病毒种。据我们所知，这是首次从土耳其沙蝇中分离出与萨勒哈巴德病毒相关的白蛉病毒并对其进行完整基因组特征分析的报告，且在白蛉病毒一直在传播并引发疫情的地中海、爱琴海和安纳托利亚中部地区有血清阳性支持。萨勒哈巴德种病毒通常被认为是一类医学或兽医关注度较低的病毒。根据我们的结果，这种观点值得重新审视，我们的结果表明阿达纳病毒的动物感染率很高，并且最近有希腊人感染阿德里亚病毒的证据。
