Buczkowska-Radlińska Jadwiga, Pol Justyna, Szmidt Monika, Bińczak-Kuleta Agnieszka
Pomeranian Medical University, Department of Conservative Dentistry.
Postepy Hig Med Dosw (Online). 2012 Apr 19;66:204-9. doi: 10.5604/17322693.991490.
The aim of the study was to analyze polymorphism of the MUC7 gene and its correlation with the DMFT value and the Plaque Control Record by O'Leary.
MATERIAL/METHODS: The study was carried out on 158 students of a faculty of dentistry in Poland. Students were subjected to a clinical oral examination. The status of caries was determined using the decayed, missing and filled teeth (DMFT) value. The status of dental hygiene was examined by the Plaque Control Record (PCR Plaque Index by O'Leary T, Drake R, Naylor, 1972) index. Sherlock AX, a universal kit for DNA isolation from biological tracks (A&A BIOTECHNOLOGY), was used for DNA isolation. VNTR polymorphism in the MUC7 gene was examined by polymerase chain reaction (PCR).
The prevalence of the MUC7*6/6 genotype was definitely higher than MUC75/6. The distribution of prevalence of MUC76/6 and MUC75/6 in the control group was similar to another. The distribution of the value of the DMFT index in the group examined with MUC76/6 was similar to the group with MUC75/*6. Statistical analysis did not show a significant correlation between genotypes of the MUC7 gene and DMFT and the Plaque Control Record index.
This study does not show a correlation between the MUC7 genotypes and caries and oral hygiene of students.
本研究旨在分析MUC7基因的多态性及其与DMFT值和O'Leary菌斑控制记录的相关性。
材料/方法：该研究在波兰一所牙科学院的158名学生中进行。对学生进行了临床口腔检查。使用龋失补牙（DMFT）值确定龋齿状况。通过菌斑控制记录（O'Leary T、Drake R、Naylor于1972年提出的PCR菌斑指数）指数检查口腔卫生状况。使用Sherlock AX（一种用于从生物样本中分离DNA的通用试剂盒，A&A BIOTECHNOLOGY公司生产）进行DNA分离。通过聚合酶链反应（PCR）检测MUC7基因中的VNTR多态性。
MUC7*6/6基因型的患病率明显高于MUC75/6。对照组中MUC76/6和MUC75/6患病率的分布彼此相似。MUC76/6检测组的DMFT指数值分布与MUC75/*6检测组相似。统计分析未显示MUC7基因的基因型与DMFT以及菌斑控制记录指数之间存在显著相关性。
本研究未显示MUC7基因型与学生龋齿及口腔卫生之间存在相关性。
