Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China,
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Int J Nanomedicine. 2018 Jul 17;13:4145-4155. doi: 10.2147/IJN.S167035. eCollection 2018.
Scaffold structure plays a vital role in cell behaviors. Compared with two-dimensional structure, 3D scaffolds can mimic natural extracellular matrix (ECM) and promote cell-cell and cell-matrix interactions. The combination of osteoconductive scaffolds and osteoinductive growth factors is considered to have synergistic effects on bone regeneration.
In this study, core-shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)-BMP-2 (PP-B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP-B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected.
The results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP-B group.
Our findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering.
支架结构在细胞行为中起着至关重要的作用。与二维结构相比，3D 支架可以模拟天然细胞外基质（ECM），促进细胞-细胞和细胞-基质相互作用。骨传导支架和骨诱导生长因子的结合被认为对骨再生具有协同作用。
本研究通过同轴静电纺丝制备了核壳型聚（乳酸-共-乙醇酸）（PLGA）/聚己内酯（PCL）-BMP-2（PP-B）纤维支架。然后，我们采用热诱导自聚集（TISA）方法，以 PP-B 纤维为基础制备了 3D 支架，并与传统的 PLGA/PCL 支架在支架形态和 BMP-2 释放行为方面进行了比较。然后，将大鼠脂肪来源干细胞（rADSCs）接种在支架上，检测 rADSCs 的增殖、细胞形态和成骨分化情况。
结果表明，负载 BMP-2 的 3D 支架显著促进了 rADSCs 的增殖和成骨分化，其次是 PP-B 组。
我们的研究结果表明，具有 3D 结构和骨诱导生长因子的支架在骨组织工程中有很大的应用潜力。
