Department of Biology, Maynooth University, Co. Kildare, Ireland.
Pathog Dis. 2018 Jun 1;76(4). doi: 10.1093/femspd/fty032.
A potential role for bacteria in the induction of rosacea has been suggested. The aim of this work was to characterise the effect of temperature on the production of immunostimulatory proteins by Bacillus oleronius-a bacterium to which rosacea patients show sera reactivity and which was originally isolated from a Demodex mite from a rosacea patient. The affected skin of rosacea patients is at a higher temperature than unaffected skin, and it was postulated that this might alter the protein expression pattern of B. oleronius. B. oleronius growth was reduced at 37°C compared to 30°C but resulted in increased expression of the immune-reactive 62kDa protein (1.65 fold [P < 0.05]). Proteomic analysis revealed increased abundance of a wide range of proteins involved in the stress response (e.g. stress proteins [21.7-fold increase], phosphocarrier protein HPr [438.5-fold increase], 60 kDa chaperonin [12.6-fold increase]). Proteins decreased in abundance after growth at 37°C included ferredoxin (325-fold decrease) and peptidase (244-fold decrease). This work indicates that the increased skin temperature of rosacea patients may alter the growth and protein production pattern of B. oleronius and lead to the greater production of immuo-stimulatory proteins.
已经有人提出细菌在引起酒渣鼻方面可能发挥作用。本研究的目的是研究温度对小菜蛾奥利昂芽孢杆菌（一种对酒渣鼻患者血清有反应的细菌，最初是从酒渣鼻患者的毛囊蠕形螨中分离出来的）产生免疫刺激性蛋白质的影响。酒渣鼻患者的患病皮肤温度高于未患病皮肤，有人推测这可能会改变 B. oleronius 的蛋白质表达模式。与 30°C 相比，37°C 时 B. oleronius 的生长减少，但导致免疫反应性 62kDa 蛋白的表达增加（1.65 倍[P < 0.05]）。蛋白质组学分析显示，涉及应激反应的多种蛋白质的丰度增加（例如应激蛋白[增加 21.7 倍]、磷酸载体蛋白 HPr[增加 438.5 倍]、60kDa 伴侣蛋白[增加 12.6 倍]）。在 37°C 下生长后减少的蛋白质包括铁氧还蛋白（减少 325 倍）和肽酶（减少 244 倍）。这项工作表明，酒渣鼻患者皮肤温度升高可能会改变 B. oleronius 的生长和蛋白质产生模式，并导致免疫刺激性蛋白质产生增加。
