Department of Genetics and Developmental Biology, Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06030, USA.
FEBS Lett. 2012 Aug 14;586(17):2586-96. doi: 10.1016/j.febslet.2012.04.019. Epub 2012 Apr 21.
The ability of modular protein domains to independently fold and bind short peptide ligands both in vivo and in vitro has allowed a significant number of protein-protein interaction studies to take advantage of them as affinity and detection reagents. Here, we refer to modular domain based proteomics as "domainomics" to draw attention to the potential of using domains and their motifs as tools in proteomics. In this review we describe core concepts of domainomics, established and emerging technologies, and recent studies by functional category. Accumulation of domain-motif binding data should ultimately provide the foundation for domain-specific interactomes, which will likely reveal the underlying substructure of protein networks as well as the selectivity and plasticity of signal transduction.
模块化蛋白结构域能够独立折叠并结合短肽配体，无论是在体内还是体外，这使得许多蛋白质-蛋白质相互作用的研究都能够利用它们作为亲和和检测试剂。在这里，我们将基于模块化结构域的蛋白质组学称为“结构域组学”，以引起人们对使用结构域及其基序作为蛋白质组学工具的关注。在这篇综述中，我们描述了结构域组学的核心概念、已建立和新兴的技术，以及按功能类别进行的最新研究。积累结构域-基序结合数据最终应该为特定结构域的互作组学提供基础，这可能会揭示蛋白质网络的潜在亚结构以及信号转导的选择性和可塑性。
