Kachel V, Messerschmidt R, Hummel P
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Cytometry. 1990;11(7):805-12. doi: 10.1002/cyto.990110706.
An 8-parameter flow cytometric data system is described using an IBM-AT compatible personal computer (PC) and a commercial analog to digital conversion (ADC) board. A dedicated pulse processing interface adapts the flow cytometric pulses to the ADC board and controls the number of parameters to be taken up and the trigger conditions. The trigger thresholds are automatically held at a level immediately above the noise level. For the timing of kinetic measurements a linear voltage ramp of adjustable rise time is available. A built-in precision voltage source can be used for an overall calibration. The data system is operated by software written in assembly language. Data may be collected and processed in 1-8-parameter listmode or 1-3-parameter histogram mode. Functions are available for graphical color displays, numerical integration, multiparameter gating, and printing.
描述了一种使用IBM-AT兼容个人计算机（PC）和商用模数转换（ADC）板的八参数流式细胞术数据系统。一个专用的脉冲处理接口使流式细胞术脉冲适配到ADC板，并控制要采集的参数数量和触发条件。触发阈值自动保持在刚好高于噪声水平的电平。对于动力学测量的定时，有一个上升时间可调的线性电压斜坡可用。内置的精密电压源可用于整体校准。该数据系统由用汇编语言编写的软件操作。数据可以在1 - 8参数列表模式或1 - 3参数直方图模式下采集和处理。具有图形彩色显示、数值积分、多参数选通和打印功能。
