Kachel V, Schneider H, Bauer J, Malin-Berdel J
Cytometry. 1983 Jan;3(4):244-50. doi: 10.1002/cyto.990030403.
Flow cytometry, usually applied to cells which have time independent features, can also be used for kinetic experiments where the change of cell populations with time is investigated. Dedicated time sequencing programs written in Assembler and incorporated in the CYTOMIC 12 analyzer (4) are described. A sequence of 64 one parameter histograms can be automatically acquired and immediately displayed as a pseudo-two-parameter histogram. The acquisition time for each of the subsequent histograms can be selected between 1 and 32 seconds. Kinetics lasting up to 34 minutes are resolved into 64 time intervals. Two parameter kinetics can be resolved into 12 32 X 32 channel, two parameter histograms which are displayed and evaluated immediately on the analyzer screen in groups of 4 without using complicated list mode procedures. The standard CYTOMIC 12 software can be applied for processing and printing of the sequence distribution curves.
流式细胞术通常应用于具有时间无关特征的细胞，也可用于研究细胞群体随时间变化的动力学实验。本文描述了用汇编语言编写并集成在CYTOMIC 12分析仪（4）中的专用时间排序程序。可以自动采集64个单参数直方图序列，并立即显示为伪双参数直方图。后续每个直方图的采集时间可在1至32秒之间选择。持续长达34分钟的动力学被分解为64个时间间隔。双参数动力学可分解为12个32×32通道的双参数直方图，这些直方图在分析仪屏幕上以4组的形式立即显示和评估，无需使用复杂的列表模式程序。标准的CYTOMIC 12软件可用于处理和打印序列分布曲线。
