Stewart S S, Price G B
Cytometry. 1986 Jan;7(1):82-8. doi: 10.1002/cyto.990070112.
A microprocessor-based system which performs realtime correlated acquisition, storage and display of multiparameter (3-parameter) data from a flow cytometer (FACS-III) is presented. List-mode techniques are not employed. The 3-parameter data is collected and correlated, then displayed along with cell-frequency as a realtime 3-parameter colour scattergram, while the experiment is in progress; in addition, correlated and uncorrelated higher-resolution projections of the 3-parameter data are collected and stored. The data projections may also be displayed: as 1-parameter histograms, or as 2-parameter colour or grey-scale scattergrams. Examples of 2- and 3-parameter colour scattergrams are presented. The speed and some characteristics of the realtime acquisition and display software are examined; methods to increase the realtime speed are discussed.
本文介绍了一种基于微处理器的系统，该系统可对来自流式细胞仪（FACS-III）的多参数（三参数）数据进行实时相关采集、存储和显示。未采用列表模式技术。在实验进行过程中，三参数数据被收集并进行关联，然后与细胞频率一起作为实时三参数彩色散点图显示；此外，还收集并存储了三参数数据的相关和不相关高分辨率投影。数据投影也可以显示为单参数直方图，或双参数彩色或灰度散点图。文中给出了双参数和三参数彩色散点图的示例。研究了实时采集和显示软件的速度及一些特性；讨论了提高实时速度的方法。
