光遗传学沉默策略在抑制性突触传递中的作用不同。
Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.
机构信息
Department of Pharmacology, Oxford University, Oxford, UK.
出版信息
Nat Neurosci. 2012 Jun 24;15(8):1102-4. doi: 10.1038/nn.3143.
Abstract
Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.
摘要
光驱动离子流的光遗传沉默允许快速有效地抑制神经活动。我们使用啮齿动物海马神经元发现，用氯离子泵进行的沉默活性在光激活后可以增加突触诱发的尖峰的概率；用质子泵则不会发生这种情况。这种效应可以通过 GABA(A)受体反转电位的变化来解释，并证明了沉默策略之间的一个重要差异。
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