Research Institute for Internal Medicine, University of Oslo, Oslo, Norway.
BMC Infect Dis. 2012 Jun 25;12:144. doi: 10.1186/1471-2334-12-144.
The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species.
Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR.
Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation.
The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA.
本研究旨在探讨慢性坏死性肺曲霉病（CNPA）患者的炎症和止血介质，CNPA 是一种由曲霉属物种侵袭引起的肺部局部破坏性疾病。
采用酶联免疫吸附试验（ELISA）和多重方法对 10 例 CNPA 患者和 19 例健康匹配对照者进行了选定生物标志物的测量。采用实时定量 RT-PCR 分析了相关生物标志物的基因表达。
CNPA 患者循环炎症介质白细胞介素（IL）-6、IL-8、RANTES、TNF-α、ICAM-1 和参与血管内皮激活和血栓形成的介质（vWF、TF 和 PAI-1）的浓度升高。抗炎细胞因子 IL-10 的浓度在患者群体的血浆和 PBMC 中均增加。PBMC 中的 CD40L 基因表达降低，同时循环中可溶性（s）CD40L 的浓度降低。
针对曲霉的促炎反应可能被降低的 CD40L 和 sCD40L 以及增加的 IL-10 所抵消，这可能会损害 CNPA 患者对曲霉的免疫反应。
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