Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Molecules. 2012 Jun 25;17(7):7758-68. doi: 10.3390/molecules17077758.
Rubus parvifolius L. (Rp) is a medicinal herb that possesses antibacterial activity. In this study, we extracted the volatile oil from the leaves of Rp to assess its antibacterial activity and analyze its chemical composition. A uniform distribution design was used to optimize the extraction procedure, which yielded 0.36% (w/w) of light yellowish oil from the water extract of Rp leaves. We found that the extracted oil effectively inhibited the growth of a wide range of Gram positive and negative bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumanii, Bacillus cloacae, and Klebsiella pneumoniae. We further analyzed the components contained in the hydro-distillated Rp volatile oil by gas chromatography-mass spectroscopy. Twenty nine compounds were identified, including 4-hydroxy-3-methoxystyrene (66%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10%) and 4-tert-butylbenzoic acid (2%). Our results suggest that one or multiple constituents contained in Rp volatile oil may account for its antibacterial activity.
悬钩子（Rp）是一种药用草本植物，具有抗菌活性。在这项研究中，我们从悬钩子叶中提取挥发油，以评估其抗菌活性并分析其化学成分。采用均匀分布设计优化了提取工艺，从悬钩子叶的水提物中得到了 0.36%（w/w）的浅黄色油。我们发现提取的油能有效抑制多种革兰氏阳性和阴性菌的生长，包括金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、大肠杆菌、铜绿假单胞菌、鲍曼不动杆菌、阴沟肠杆菌和肺炎克雷伯菌。我们进一步通过气相色谱-质谱分析了水蒸馏悬钩子挥发油中所含的成分。鉴定出 29 种化合物，包括 4-羟基-3-甲氧基苯乙烯（66%）、3,7,11,15-四甲基-2-十六碳烯-1-醇（10%）和 4-叔丁基苯甲酸（2%）。我们的结果表明，悬钩子挥发油中的一种或多种成分可能是其抗菌活性的原因。
