Balasubramaniam N K, Veerisetty V, Gentry G A
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216.
J Gen Virol. 1990 Dec;71 ( Pt 12):2979-87. doi: 10.1099/0022-1317-71-12-2979.
Twelve herpesviral deoxythymidine kinases were examined for regions of sequence similarity by multiple alignment. Six highly conserved sites were observed. Site 1 corresponded to a glycine-rich loop that forms part of the ATP-binding pocket in porcine adenylate kinase (PAK), and site 5 corresponded to a region in PAK, located on one lobe of the cleft, that contains arginine residues that bind substrate phosphoryl groups. Site 3, consisting of the motif -DRH-, is thought to be involved in thymine/deoxythymidine recognition; site 4, which is nearby, probably participates in this function as well. The functions of sites 2 and 6 have not been identified. Secondary structure predictions were made by the Garnier method and averaged for each position in the multiple alignment. The structure predicted for all six sites was typically a short flexible region (turn or coil) at or adjacent to the site, flanked by rigid structures (helix or sheet) on either side.
通过多重比对，对12种疱疹病毒脱氧胸苷激酶的序列相似区域进行了检测。观察到6个高度保守的位点。位点1对应于猪腺苷酸激酶（PAK）中构成ATP结合口袋一部分的富含甘氨酸的环，位点5对应于PAK中位于裂隙一个叶上的区域，该区域含有结合底物磷酰基的精氨酸残基。由基序-DRH-组成的位点3被认为参与胸腺嘧啶/脱氧胸苷识别；附近的位点4可能也参与此功能。位点2和6的功能尚未确定。通过 Garnier 方法进行二级结构预测，并对多重比对中每个位置的预测结果求平均值。预测的所有6个位点的结构通常是该位点处或相邻处的短柔性区域（转角或卷曲），两侧为刚性结构（螺旋或片层）。
