Milles Sigrid, Koehler Christine, Gambin Yann, Deniz Ashok A, Lemke Edward A
EMBL, Structural and Computational Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Mol Biosyst. 2012 Oct;8(10):2531-4. doi: 10.1039/c2mb25135c.
Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.
荧光共振能量转移的单分子观察可用于深入了解蛋白质的结构和动力学。通过一种简单的三色标记策略，我们提出了一种测量和分析方案，该方案可以同时研究单个内在无序蛋白质中的多个区域。
