Department of Biology, University of Richmond, 28 Westhampton Way, Richmond, VA 23173, USA.
Am J Bot. 2012 Jul;99(7):e298-300. doi: 10.3732/ajb.1100612. Epub 2012 Jun 27.
Novel microsatellite primers were developed for the native wildflower Ipomopsis aggregata to facilitate ongoing studies of the genetics of local adaptation and patterns of hybridization with closely related species within the genus.
Thirteen primer sets were successfully developed and tested using populations from the Rocky Mountains of Colorado, USA. The primers amplified di-, tri-, and tetranucleotide repeats with 1-15 alleles per locus. All primers also amplified fragments with varying success in closely related Ipomopsis species and more distant members of the Polemoniaceae.
The polymorphism levels observed across all loci suggest that these microsatellites may be useful for population genetic studies in Ipomopsis, as well as in studies of other related taxa in the Polemoniaceae.
为了方便对本地适应性的遗传学和与属内近缘种杂交模式的研究，我们为原生野花山梗菜开发了新的微卫星引物。
在美国科罗拉多落矶山脉的种群中，我们成功地开发和测试了 13 组引物。这些引物扩增了二、三、四核苷酸重复序列，每个位点有 1-15 个等位基因。所有的引物在亲缘关系较近的山梗菜属物种和更远缘的茄科成员中也都能不同程度地扩增出片段。
所有位点观察到的多态性水平表明，这些微卫星标记可能对山梗菜属的种群遗传研究以及茄科其他相关分类群的研究都有用。
