Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.
J Sep Sci. 2012 Jun;35(12):1462-9. doi: 10.1002/jssc.201200135.
A new model of solvent gradients selection was rationally developed for the preparative separation of target compounds. The solvent gradients were selected based on a three-stage screening process where stationary phase retention was ensured by introducing a new parameter termed as the phase ratio. The phase ratio was calculated after mixing the upper phase of a solvent system with the lower phase of a different solvent system (1:1, v/v). The developed model was applied to the one-step separation of eight ginsenosides from Panax ginseng. Three gradients were selected on the basis of new model and eight ginsenosides, Rb(1), Rb(2), Rc, Rd, Re, Rg(1), Rf, and Rh(1), were efficiently separated by high-speed counter-current chromatography coupled with evaporative light scattering detector. The structures of all compounds were characterized by electrospray-ionization mass spectrometry and nuclear magnetic resonance spectroscopy.
合理开发了一种新的溶剂梯度选择模型，用于目标化合物的制备性分离。溶剂梯度是基于三阶段筛选过程选择的，其中通过引入称为相比的新参数来确保固定相保留。相比是在将溶剂系统的上相与不同溶剂系统的下相（1:1，v/v）混合后计算得出的。该模型应用于从人参中一步分离八种人参皂苷。根据新模型和八种人参皂苷选择了三种梯度，通过高速逆流色谱法与蒸发光散射检测器联用，高效分离了 Rb(1)、Rb(2)、Rc、Rd、Re、Rg(1)、Rf 和 Rh(1)。所有化合物的结构均通过电喷雾电离质谱和核磁共振波谱进行了表征。
