尸检后时间延迟对恒河猴潜伏感染神经节中猴痘 - 带状疱疹病毒表达分析的影响。
Effect of time delay after necropsy on analysis of simian varicella-zoster virus expression in latently infected ganglia of rhesus macaques.
机构信息
Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA.
出版信息
J Virol. 2010 Dec;84(23):12454-7. doi: 10.1128/JVI.01792-10. Epub 2010 Sep 22.
Abstract
Studies of varicella-zoster virus gene expression during latency require the acquisition of human ganglia at autopsy. Concerns have been raised that the virus might reactivate immediately after death. Because features of varicella-zoster virus latency are similar in primate and human ganglia, we examined virus gene expression in tissues either processed immediately or kept at 4°C for 30 h before necropsy of two monkeys inoculated with simian varicella-zoster virus and euthanized 117 days later. Virus transcription and the detection of open reading frame (ORF) 63 protein in the cytoplasm of neurons were comparable. Thus, a 30-h delay after death did not affect varicella-zoster virus expression in latently infected ganglia.
摘要
研究潜伏性水痘带状疱疹病毒基因表达需要在尸检时获取人神经节。人们担心病毒可能会在死亡后立即重新激活。由于灵长类动物和人类神经节中的水痘带状疱疹病毒潜伏特征相似，我们检测了两只因感染猴水痘-带状疱疹病毒而接受安乐死的猴子的组织，这些猴子在接种后 117 天死亡，这些组织要么在死后立即处理，要么在 4°C 下保存 30 小时后进行尸检。病毒转录和细胞质中开放阅读框（ORF）63 蛋白的检测结果相似。因此，死后 30 小时的延迟不会影响潜伏感染神经节中的水痘带状疱疹病毒表达。
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