人类三叉神经节中潜伏性水痘带状疱疹病毒和单纯疱疹病毒基因组的定量分析。
Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia.
作者信息
Pevenstein S R, Williams R K, McChesney D, Mont E K, Smialek J E, Straus S E
机构信息
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
出版信息
J Virol. 1999 Dec;73(12):10514-8. doi: 10.1128/JVI.73.12.10514-10518.1999.
Abstract
Using real-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (53%) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 +/- 1,082 (standard error of the mean) or 109 genomes/10(5) cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 +/- 38 genomes/10(5) ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.
摘要
我们运用实时荧光聚合酶链反应（PCR），对15例人类三叉神经节中潜伏的水痘-带状疱疹病毒（VZV）以及单纯疱疹病毒1型（HSV-1）和2型（HSV-2）基因组的拷贝数进行了定量分析。15个神经节中，分别有8个（53%）和1个（7%）的HSV-1或-2糖蛋白G基因PCR检测呈阳性，其平均值分别为2,902±1,082（均值标准误）或109个基因组/10⁵个细胞。14个神经节中的11个（79%）至15个神经节中的13个（87%），其VZV基因29、31或62的PCR检测呈阳性。将这三个VZV基因的结果汇总后，得出的平均值为258±38个基因组/10⁵个神经节细胞。这些潜伏病毒基因组负荷水平对病毒在人类感觉神经节中的分布及再激活具有重要意义。
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