Li Zhenyang, Gu Xiaodong, Fang Yantian, Xiang Jianbin, Chen Zongyou
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
Oncol Lett. 2012 Feb;3(2):346-350. doi: 10.3892/ol.2011.497. Epub 2011 Nov 29.
The present study aimed to identify microRNA (miRNA) expression profiles associated with brain metastases of colorectal cancers. We conducted miRNA expression profiling of 3 primary colorectal cancers and 3 brain-metastatic carcinomas using Agilent Human miRNA Microarrays. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was employed to validate the results obtained from the miRNA microarray analysis. Overexpression of miR-145, miR-1, miR-146a, miR-576-5p, miR-126*, HS287, miR-28-5p, miR-143, miR-199b-5p, miR-199a-5p, miR-10b, miR-22, miR-133b, miR-145*, miR-199a, miR-133a, miR-125b and downregulation of miR-31 and HS170 were observed in brain-metastatic carcinomas. Quantitative RT-PCR experiments with miR-125b confirmed the expression patterns we found in our microarray experiments. miRNAs are differentially expressed between colorectal cancers and matched brain-metastatic carcinomas. The miRNA variation trend is quite different in the process of metastasis compared to that of carcinogenesis. These miRNAs may therefore serve as potential diagnostic markers and therapeutic targets for colorectal cancers with brain metastases.
本研究旨在鉴定与结直肠癌脑转移相关的微小RNA（miRNA）表达谱。我们使用安捷伦人类miRNA微阵列对3例原发性结直肠癌和3例脑转移癌进行了miRNA表达谱分析。采用定量实时逆转录-聚合酶链反应（RT-PCR）来验证从miRNA微阵列分析中获得的结果。在脑转移癌中观察到miR-145、miR-1、miR-146a、miR-576-5p、miR-126*、HS287、miR-28-5p、miR-143、miR-199b-5p、miR-199a-5p、miR-10b、miR-22、miR-133b、miR-145*、miR-199a、miR-133a、miR-125b的过表达以及miR-31和HS170的下调。对miR-125b进行的定量RT-PCR实验证实了我们在微阵列实验中发现的表达模式。miRNA在结直肠癌和匹配的脑转移癌之间存在差异表达。与致癌过程相比，miRNA在转移过程中的变化趋势有很大不同。因此，这些miRNA可能作为结直肠癌脑转移的潜在诊断标志物和治疗靶点。
