Department of Bioinformatics, MMV, Banaras Hindu University, Varanasi 221 005, India.
J Biosci. 2012 Jul;37(3):563-71. doi: 10.1007/s12038-012-9208-4.
Enhanced disease susceptibility 1 (EDS1), a plant-specific protein has homology with the eukaryotic lipase in their N-terminal halves and a unique domain at its C-termini. EDS1 is known to be an important regulator of biotic stress and an essential component of basal immunity. EDS1 interacts with its positive co-regulator phytoalexin deficient 4 (PAD4), resulting in mobilization of the salicylic acid defence pathway. Limited information regarding this interaction in rice is available. To study this interaction, a model of EDS1 and PAD4 proteins from rice was generated and validated with Accelrys DS software version 3.1 using bioinformatics interface. The in silico docking between the two proteins showed a significant protein-protein interaction between rice EDS1 and PAD4, suggesting that they form a dimeric protein complex, which, similar to that in Arabidopsis, is perhaps also important for triggering the salicylic acid signalling pathway in plants.
增强的疾病易感性 1（EDS1）是一种植物特异性蛋白，其 N 端的前半部分与真核脂肪酶具有同源性，而 C 端则具有独特的结构域。EDS1 已知是生物胁迫的重要调节剂，也是基础免疫的重要组成部分。EDS1 与它的正调控因子phytoalexin deficient 4（PAD4）相互作用，导致水杨酸防御途径的激活。关于水稻中这种相互作用的信息有限。为了研究这种相互作用，我们从水稻中生成了 EDS1 和 PAD4 蛋白的模型，并使用 Accelrys DS 软件版本 3.1 通过生物信息学接口进行了验证。这两种蛋白的计算机对接显示，水稻 EDS1 和 PAD4 之间存在显著的蛋白-蛋白相互作用，表明它们形成了二聚体蛋白复合物，与拟南芥中的复合物类似，这对于植物中水杨酸信号通路的触发也可能很重要。
