Singh Prerna, Singh Indra, Shah Kavita
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, India.
Faculty of Science, School of Biotechnology, Banaras Hindu University, Varanasi, India.
Front Plant Sci. 2019 Jan 15;9:1948. doi: 10.3389/fpls.2018.01948. eCollection 2018.
Cadmium is a well known toxic heavy metal, which has various detrimental effects on plant system. In plants an important enzyme involved in the production of nitric oxide, nitrate reductase, is also affected by cadmium toxicity. According to many studies cadmium has an inhibitory effect on nitrate reductase activity. Similar effect of cadmium was found in our study where an inhibitory effect of cadmium on nitrate reductase activity was noted. However, the mechanism behind this inhibition has not been explored. With the help of homology, 3-D structure of rice-nitrate reductase is modeled in this study. Its binding with nitrate, nitrite and cadmium metal has been explored. The bonds formed between the enzyme-substrate complex, enzyme-cadmium and differences in interactions in presence of cadmium has been studied in detail. The present study should help in understanding the modeled structure of rice-nitrate reductase in 3-D which may in turn guide enzyme related studies . The present study also provides an insight as to how cadmium interacts with nitrate reductase to alter the enzyme activity.
镉是一种众所周知的有毒重金属，对植物系统有多种有害影响。在植物中，一种参与一氧化氮生成的重要酶——硝酸还原酶，也会受到镉毒性的影响。根据许多研究，镉对硝酸还原酶活性有抑制作用。在我们的研究中也发现了镉的类似作用，即镉对硝酸还原酶活性有抑制作用。然而，这种抑制背后的机制尚未被探索。在本研究中，借助同源性对水稻硝酸还原酶的三维结构进行了建模。研究了它与硝酸盐、亚硝酸盐和镉金属的结合情况。详细研究了酶 - 底物复合物、酶 - 镉之间形成的键以及镉存在时相互作用的差异。本研究应有助于理解水稻硝酸还原酶的三维建模结构，这反过来可能会指导与该酶相关的研究。本研究还深入探讨了镉如何与硝酸还原酶相互作用以改变酶的活性。
