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用于优化天然菌株SF3生产右旋糖酐的析因设计。

Factorial Design to Optimize Dextran Production by the Native Strain SF3.

作者信息

Yáñez-Fernández Jorge, Herrera Ovando Mirna Griselda, Patlán Ramírez Larissa, Ramírez-Sotelo Guadalupe, Guarin Cesar A, Castro-Rodríguez Diana C

机构信息

Unidad profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional (IPN), Mexico City 07340, Mexico.

Universidad Autónoma Metropolitana-Iztapalapa, Mexico City 09340, Mexico.

出版信息

ACS Omega. 2021 Nov 9;6(46):31203-31210. doi: 10.1021/acsomega.1c04856. eCollection 2021 Nov 23.

Abstract

Dextran is an extracellular bacterial polysaccharide for which industrial applications have been found in different areas. Several researchers have optimized the fermentation conditions to maximize dextran production. This study aimed to characterize the dextran of SF3, which was isolated from the aguamiel of . To maximize the yield of dextran, the effects of sucrose concentration, temperature, and incubation time were studied. The experiments were conducted using a factorial design and a response surface methodology. SF3 produced a maximum yield of dextran (23.8 g/L ± 4) after 16 h of incubation at 25 °C with 10% sucrose. The functional properties such as water absorption capacity, oil absorption capacity, and emulsion activity of this unique dextran were 361.8% ± 3.1, 212.0% ± 6.7, and 58.3% ± 0.7, respectively. These properties indicate that the dextran produced by SF3 is a high-quality polysaccharide with potential applications in the food industry, and the optimized conditions for its production could be used for the commercial production of this dextran, which have significant industrial perspectives.

摘要

右旋糖酐是一种细胞外细菌多糖,已在不同领域发现其工业应用。几位研究人员对发酵条件进行了优化,以实现右旋糖酐产量最大化。本研究旨在表征从[此处信息缺失]的龙舌兰汁液中分离出的SF3的右旋糖酐。为使右旋糖酐产量最大化,研究了蔗糖浓度、温度和培养时间的影响。实验采用析因设计和响应面方法进行。SF3在25℃下用10%蔗糖培养16小时后,右旋糖酐产量最高(23.8 g/L±4)。这种独特的右旋糖酐的吸水能力、吸油能力和乳化活性等功能特性分别为361.8%±3.1、212.0%±6.7和58.3%±0.7。这些特性表明,SF3产生的右旋糖酐是一种高质量的多糖,在食品工业中有潜在应用,其优化的生产条件可用于这种右旋糖酐的商业生产,具有重要的工业前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31bb/8613848/10a0b3033d2f/ao1c04856_0002.jpg

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