Aranda Jesús, Teixidó Laura, Fittipaldi Nahuel, Cortés Pilar, Llagostera Montserrat, Gottschalk Marcelo, Barbé Jordi
Department de Genètica i Microbiologia, Edifici C (Facultat de Biociències), Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallès), 08193 Barcelona, Spain.
Can J Vet Res. 2012 Jan;76(1):72-6.
The Streptococcus suis 103 gene product is an immunogenic and protective lipoprotein that is a component of an ATP-binding cassette transporter implicated in zinc uptake. Belonging to the same transcriptional unit and downstream of the 103 gene is a gene that encodes a homologue of the pneumococcal histidine triad (Pht) protein Pht309. In an intraperitoneal mouse model the virulence of a mutant lacking the 103 gene was more than 50 times lower than that of the wild-type (WT) parent strain, S. suis serotype 2 strain P1/7. In addition, the immunogenicity of this mutant was dramatically decreased. In striking contrast, the virulence and immunogenicity of a P1/7 mutant lacking the Pht309 gene were similar to those of the parent strain. These results demonstrate that the 103 lipoprotein is strongly involved in S. suis virulence and support the hypothesis that this lipoprotein might be an excellent candidate for vaccines aiming to achieve broad protection against streptococci.
猪链球菌103基因产物是一种具有免疫原性和保护性的脂蛋白，是参与锌摄取的ATP结合盒转运体的一个组成部分。与103基因属于同一转录单元且位于其下游的是一个编码肺炎球菌组氨酸三联体（Pht）蛋白Pht309同源物的基因。在腹腔注射小鼠模型中，缺失103基因的突变体的毒力比野生型（WT）亲本菌株猪链球菌2型菌株P1/7低50倍以上。此外，该突变体的免疫原性显著降低。与之形成鲜明对比的是，缺失Pht309基因的P1/7突变体的毒力和免疫原性与亲本菌株相似。这些结果表明，103脂蛋白强烈参与猪链球菌的毒力，并支持这样一种假设，即这种脂蛋白可能是旨在实现对链球菌广泛保护的疫苗的优秀候选物。
