Lizcano Leandro J, Viloria-Bernal María, Vicente Francisca, Berrueta Luis Angel, Gallo Blanca, Martínez-Cañamero Magdalena, Ruiz-Larrea Maria Begoña, Ruiz-Sanz José Ignacio
Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain.
Department of Analytical Chemistry, Sciences and Technology School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain.
Int J Mol Sci. 2012;13(5):5454-5467. doi: 10.3390/ijms13055454. Epub 2012 May 4.
Diverse plants of ethnobotanic interest in Amazonia are commonly used in traditional medicine. We determined the antioxidant potential against lipid peroxidation, the antimicrobial activity, and the polyphenol composition of several Amazonian plants (Brownea rosademonte, Piper glandulosissimum, Piper krukoffii, Piper putumayoense, Solanum grandiflorum, and Vismia baccifera). Extracts from the plant leaf, bark, and stem were prepared as aqueous infusions, as used in folk medicine, and added to rat liver microsomes exposed to iron. The polyphenolic composition was detected by reverse-phase HPLC coupled to diode-array detector and MS/MS analysis. The antimicrobial activity was tested by the spot-on-a-lawn method against several indicator microorganisms. All the extracts inhibited lipid oxidation, except the P. glandulosissimum stem. The plant extracts exhibiting high antioxidant potential (V. baccifera and B. rosademonte) contained high levels of flavanols (particularly, catechin and epicatechin). By contrast, S. grandiflorum leaf, which exhibited very low antioxidant activity, was rich in hydroxycinnamic acids. None of the extracts showed antimicrobial activity. This study demonstrates for the first time the presence of bioactive polyphenolic compounds in several Amazonian plants, and highlights the importance of flavanols as major phenolic contributors to antioxidant activity.
亚马逊地区多种具有民族植物学研究价值的植物常用于传统医学。我们测定了几种亚马逊植物（红苞 Brownea rosademonte、腺叶胡椒 Piper glandulosissimum、克鲁科夫氏胡椒 Piper krukoffii、普图马约胡椒 Piper putumayoense、大花茄 Solanum grandiflorum 和多花粘木 Vismia baccifera）对脂质过氧化的抗氧化潜力、抗菌活性及多酚成分。按照民间医学的用法，将植物叶、树皮和茎的提取物制成水浸剂，并添加到暴露于铁的大鼠肝微粒体中。通过反相高效液相色谱结合二极管阵列检测器和串联质谱分析检测多酚成分。采用琼脂平板点滴法对几种指示微生物测试抗菌活性。除腺叶胡椒茎提取物外，所有提取物均能抑制脂质氧化。具有高抗氧化潜力的植物提取物（多花粘木和红苞 Brownea rosademonte）含有高水平的黄烷醇（特别是儿茶素和表儿茶素）。相比之下，抗氧化活性极低的大花茄叶富含羟基肉桂酸。所有提取物均未显示出抗菌活性。本研究首次证明了几种亚马逊植物中存在生物活性多酚化合物，并强调了黄烷醇作为抗氧化活性主要酚类成分的重要性。
