通过 ULPS-ESI-Q-oa/TOF-MS 分析豆荚中的有机和水提物中的酚类化合物。在 CD-1 小鼠中的体外抗氧化活性和抗炎反应。

Phenolic Compounds in Organic and Aqueous Extracts from Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice.

机构信息

Departamento de Nutrición Animal Dr. Fernando Pérez-Gil Romo, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), CDMX 14080, Mexico.

Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), CDMX 04510, Mexico.

出版信息

Molecules. 2018 Sep 18;23(9):2386. doi: 10.3390/molecules23092386.

BACKGROUND

(AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacity to curb the inflammation process as well as to down-regulate the pro-inflammatory mediators.

METHODS

Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessment of anti-inflammatory properties was made by the ear edema induced model in CD-1 mice and MPO activity assay. Likewise, histological analysis, IL-1β, IL-6, IL-10, TNF-α, COX measurements plus nitrite and immunohistochemistry analysis were carried out.

RESULTS

Methyl gallate, gallic acid, galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays.

CONCLUSION

All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents.

背景

（AF）豆荚传统上被用于治疗消化不良、腹泻和皮肤炎症的局部治疗。主要目的：（1）研究来自 AF 豆荚的六种提取物的抗氧化活性和对氧化诱导损伤的保护作用，以及（2）它们抑制炎症过程和下调促炎介质的能力。

方法

获得了五种有机提取物（氯仿、己烷、酮、甲醇、甲醇：水和一种水提取物），并通过 UPLC-ESI-Q-oa/TOF-MS 进行分析。进行了抗氧化活性（DPPH•、ORAC 和 FRAP 测定）和脂质过氧化（TBARS 测定）。通过 CD-1 小鼠耳水肿诱导模型和 MPO 活性测定评估抗炎特性。此外，还进行了组织学分析、IL-1β、IL-6、IL-10、TNF-α、COX 测量以及亚硝酸盐和免疫组织化学分析。

结果

鉴定出甲基没食子酸、没食子酸、葡萄糖基没食子酸 1、葡萄糖基没食子酸 2、葡萄糖基没食子酸 3、二没食子酰葡萄糖异构体 1、二没食子酰葡萄糖异构体 2、二没食子酰葡萄糖异构体 3、二没食子酰葡萄糖异构体 4、羟基酪醇乙酸酯、奎尼酸和咖啡酰苹果酸。有机和水提取物均显示出抗氧化活性。所有提取物均对白细胞介素、COX 和免疫组织化学测定产生积极影响。