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伪狂犬病:逆流免疫电泳法在检测猪血清抗体中的应用

Pseudorabies: adaptation of the countercurrent immunoelectrophoresis for the detection of antibodies in porcine serum.

作者信息

Papp-Vid G, Dulac G C

出版信息

Can J Comp Med. 1979 Apr;43(2):119-24.

PMID:227550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1319905/
Abstract

A countercurrent immunoelectrophoresis test was developed for the detection of precipitating antibodies to pseudorabies virus in pig serum. The precipitation reaction occurred only between the pseudorables antigen and the homologous porcine antiserum. The sensitivity of the method was compared to that of the serum neutralization test. On the basis of its sensitivity, its specificity and the rapidity with which the results were obtained, the countercurrent immunoelectrophoresis may become a potentially valuable screening method to test large numbers of porcine serum.

摘要

已开发出一种对流免疫电泳试验,用于检测猪血清中针对伪狂犬病病毒的沉淀抗体。沉淀反应仅发生在伪狂犬病抗原与同源猪抗血清之间。将该方法的灵敏度与血清中和试验的灵敏度进行了比较。基于其灵敏度、特异性以及获得结果的快速性,对流免疫电泳可能成为一种检测大量猪血清的潜在有价值的筛选方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2a8/1319905/9a30265f27e9/compmed00026-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2a8/1319905/9a30265f27e9/compmed00026-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2a8/1319905/9a30265f27e9/compmed00026-0008-a.jpg

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1
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引用本文的文献

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本文引用的文献

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Production de Porcs Exempts d'Organismes Pathogenes (EOPS) Primaires et Etablissement d'un Troupeau de Porcs EOPS Secondaires.初级无特定病原体猪(SPF猪)的生产及二级SPF猪群的建立。
Can J Comp Med Vet Sci. 1962 Dec;26(12):279-84.
2
Studies on Pathogenic Porcine Enteroviruses: II. Isolation of Virus in Tissue Culture from Brain and Feces of Clinical Cases.致病性猪肠道病毒的研究:II. 从临床病例的脑和粪便中通过组织培养分离病毒
Can J Comp Med Vet Sci. 1961 Jun;25(6):142-50.
3
THE DISTRIBUTION OF THE VIRUS OF AUJESZKY'S DISEASE (PSEUDORABIES VIRUS) IN EXPERIMENTALLY INFECTED SWINE.
改良对流免疫电泳试验与微量免疫扩散试验检测猪血清中伪狂犬病病毒抗体的比较。
Can J Comp Med. 1984 Jan;48(1):92-6.
4
Development and evaluation of an indirect enzyme immunoassay for detection of porcine antibodies to pseudorabies virus.用于检测猪伪狂犬病病毒抗体的间接酶免疫测定法的建立与评估
Can J Vet Res. 1986 Jul;50(3):422-6.
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Dot-enzyme immunoassay for visual detection of antibodies to pseudorabies virus in swine serum.
J Clin Microbiol. 1986 Mar;23(3):563-7. doi: 10.1128/jcm.23.3.563-567.1986.
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Evaluation of an enzyme immunoassay for detection of antibodies to pseudorabies virus in porcine field sera.用于检测猪现场血清中伪狂犬病病毒抗体的酶免疫测定法的评估
Can J Vet Res. 1987 Oct;51(4):539-41.
奥耶斯基氏病病毒(伪狂犬病病毒)在实验性感染猪体内的分布
Am J Vet Res. 1965 May;26:631-5.
4
Antigen and antibody in Aleutian disease in mink. I. Precipitation reaction by agar-gel electrophoresis.水貂阿留申病中的抗原与抗体。I. 琼脂凝胶电泳沉淀反应
J Immunol. 1972 Feb;108(2):555-7.
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Serological relationships among viruses of the herpes group.疱疹病毒组病毒之间的血清学关系。
J Gen Virol. 1967 Jan;1(1):139-41. doi: 10.1099/0022-1317-1-1-139.
6
Preliminary studies on the development of a swine vesicular disease vaccine.
Arch Gesamte Virusforsch. 1974;44(4):350-60. doi: 10.1007/BF01251016.
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In vitro method for safety testing of foot-and-mouth disease vaccines.口蹄疫疫苗安全性检测的体外方法
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