Department of Dermatology and Allergology, RWTH Aachen University, Aachen, Germany.
Skin Pharmacol Physiol. 2012;25(5):241-8. doi: 10.1159/000341144. Epub 2012 Jun 29.
Topical application of dexpanthenol is widely used in clinical practice for the improvement of wound healing. Previous in vitro experiments identified a stimulatory effect of pantothenate on migration, proliferation and gene regulation in cultured human dermal fibroblasts. To correlate these in vitro findings with the more complex in vivo situation of wound healing, a clinical trial was performed in which the dexpanthenol-induced gene expression profile in punch biopsies of previously injured and dexpanthenol-treated skin in comparison to placebo-treated skin was analyzed at the molecular level by Affymetrix® GeneChip analysis. Upregulation of IL-6, IL-1β, CYP1B1, CXCL1, CCL18 and KAP 4-2 gene expression and downregulation of psorasin mRNA and protein expression were identified in samples treated topically with dexpanthenol. This in vivo study might provide new insight into the molecular mechanisms responsible for the effect of dexpanthenol in wound healing and shows strong correlations to previous in vitro data using cultured dermal fibroblasts.
将德帕醇局部应用于临床，广泛用于改善伤口愈合。先前的体外实验表明泛酸对培养的人真皮成纤维细胞的迁移、增殖和基因调控具有刺激作用。为了将这些体外发现与更复杂的伤口愈合的体内情况相关联，进行了一项临床试验，通过 Affymetrix® GeneChip 分析，在分子水平上分析了先前受伤的和德帕醇治疗的皮肤与安慰剂治疗的皮肤的活检样本中德帕醇诱导的基因表达谱。在接受德帕醇局部治疗的样本中，发现白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、细胞色素 P4501B1（CYP1B1）、趋化因子（C-X-C）配体 1（CXCL1）、趋化因子（C-C）配体 18（CCL18）和丝聚合蛋白（KAP4-2）基因表达上调，而 psorasin mRNA 和蛋白表达下调。这项体内研究可能为德帕醇在伤口愈合中的作用的分子机制提供新的见解，并与使用培养的真皮成纤维细胞的先前体外数据具有很强的相关性。
