Department of Animal Science, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, Zagreb, Croatia.
Anim Genet. 2013 Apr;44(2):184-92. doi: 10.1111/j.1365-2052.2012.02393.x. Epub 2012 Jul 4.
Variation in mitochondrial DNA (mtDNA) and Y-chromosome haplotypes was analysed in nine domestic sheep breeds (159 rams) and 21 mouflon (Ovis musimon) sampled in the East Adriatic. Mitochondrial DNA analyses revealed a high frequency of type B haplotypes, predominantly in European breeds, and a very low frequency of type A haplotypes, which are more frequent in some Asian breeds. Mitochondrial haplotype Hmt-3 was the most frequent (26.4%), and 37.1%, 20.8% and 7.6% of rams had haplotypes one, two and three mutations remote from Hmt-3 respectively. In contrast, Y-chromosome analyses revealed extraordinary paternal allelic richness: HY-6, 89.3%; HY-8, 5.0%; HY-18, 3.1%; HY-7, 1.3%; and HY-5, 1.3%. In fact, the number of haplotypes observed is comparable to the number found in Turkish breeds and greater than the number found in European breeds so far. Haplotype HY-18 (A-oY1/135-SRYM18), identified here for the first time, provides a link between the haplotype HY-12 (A-oY1/139-SRYM18) found in a few rams in Turkey and haplotype HY-9 (A-oY1/131-SRYM18) found in one ram in Ethiopia. All mouflons had type B mtDNA haplotypes, including the private haplotype (Hmt-55), and all were paternally monomorphic for haplotype HY-6. Our data support a quite homogeneous maternal origin of East Adriatic sheep, which is a characteristic of European breeds. At the same time, the high number of haplotypes found was surprising and intriguing, and it begs for further analysis. Simultaneous analysis of mtDNA and Y-chromosome information allowed us to detect a large discrepancy between maternal and paternal lineages in some populations. This is most likely the result of breeder efforts to 'upgrade' local populations using rams with different paternal origins.
在东亚得里亚海地区，对 9 个绵羊品种（159 只公羊）和 21 只摩弗伦羊（Ovis musimon）进行了线粒体 DNA（mtDNA）和 Y 染色体单倍型的变异分析。mtDNA 分析显示 B 型单倍型的频率较高，主要存在于欧洲品种中，而 A 型单倍型的频率非常低，在一些亚洲品种中更为常见。mtDNA 单倍型 Hmt-3 最为常见（26.4%），有 37.1%、20.8%和 7.6%的公羊分别具有与 Hmt-3 相差一个、两个和三个突变的单倍型一、二和三。相比之下，Y 染色体分析显示出非凡的父系等位基因丰富度：HY-6，89.3%；HY-8，5.0%；HY-18，3.1%；HY-7，1.3%；HY-5，1.3%。事实上，观察到的单倍型数量与在土耳其品种中发现的数量相当，并且大于迄今为止在欧洲品种中发现的数量。在这里首次发现的单倍型 HY-18（A-oY1/135-SRYM18）为单倍型 HY-12（A-oY1/139-SRYM18）提供了联系，该单倍型存在于土耳其的几只公羊中，而单倍型 HY-9（A-oY1/131-SRYM18）存在于埃塞俄比亚的一只公羊中。所有的摩弗伦羊都具有 B 型 mtDNA 单倍型，包括单倍型（Hmt-55），并且所有的父系单倍型均为 HY-6。我们的数据支持东亚得里亚海绵羊具有相当同质的母系起源，这是欧洲品种的特征。同时，发现的大量单倍型令人惊讶和好奇，这需要进一步分析。mtDNA 和 Y 染色体信息的同时分析表明，在一些种群中，母系和父系谱系之间存在很大差异。这很可能是由于饲养者努力使用具有不同父系起源的公羊来“升级”当地种群的结果。
