Wang Yutao, Xu Lei, Yan Wei, Li Shaobin, Wang Jiqing, Liu Xiu, Hu Jiang, Luo Yuzhu
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashgar Teachers College, Kashgar 844000, China.
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Gene. 2015 Jul 10;565(2):242-5. doi: 10.1016/j.gene.2015.04.015. Epub 2015 Apr 9.
Investigations on the variation present at the male-specific Y chromosome region provide strong information to understand the origin and evolution of domestic sheep. One SNP OY1 (g.88A>G) in the upstream region of SRY gene, and the microsatellite SRYM18 locus within ovine Y chromosome were analyzed in one hundred and forty five samples collected from eleven breeds in China. SNP OY1 was analyzed using PCR-SSCP method and sequencing. Two different PCR-SSCP patterns represented two specific sequences with sequence analysis revealing SNP-OY1 (g.88A>G) were observed, while SNP A-OY1 showed the most common frequency (82.8%). Sequencing of the SRYM18 region revealed one novel size fragment (A2) with different repetitive units. Seven haplotypes (H4, H5, H6, H7, H8, H9 and H12) and two novel haplotypes (Ha and Hb) were established using combined genotype analysis. H6 showed the highest frequency (43.4%) across all breeds, and H8 showed the second frequency (24.1%). Ha was only found in one breed (Tan), while Hb was present in three breeds (Gansu alpine, White Suffolk and Duolang). Our findings reveal one novel allele in SRYM18 region and two novel male haplotypes of domestic sheep in China.
对雄性特异性Y染色体区域存在的变异进行研究，为了解家羊的起源和进化提供了有力信息。在中国11个品种收集的145个样本中，分析了SRY基因上游区域的一个单核苷酸多态性OY1（g.88A>G）以及绵羊Y染色体上的微卫星SRYM18位点。采用PCR-SSCP方法和测序对SNP OY1进行分析。观察到两种不同的PCR-SSCP模式代表两个特定序列，序列分析揭示了SNP-OY1（g.88A>G），而SNP A-OY1显示出最常见的频率（82.8%）。SRYM18区域的测序揭示了一个具有不同重复单元的新大小片段（A2）。使用联合基因型分析建立了7种单倍型（H4、H5、H6、H7、H8、H9和H12）和两种新单倍型（Ha和Hb）。H6在所有品种中频率最高（43.4%），H8频率次之（24.1%）。Ha仅在一个品种（滩羊）中发现，而Hb存在于三个品种（甘肃高山细毛羊、白萨福克羊和多浪羊）中。我们的研究结果揭示了SRYM18区域的一个新等位基因以及中国家羊的两种新雄性单倍型。
