脂肪组织来源的间充质干细胞作为潜伏利什曼病的新宿主细胞。
Adipose tissue-derived mesenchymal stem cells as a new host cell in latent leishmaniasis.
机构信息
Department of Bioengineering, Yildiz Technical University, Istanbul, Turkey.
出版信息
Am J Trop Med Hyg. 2011 Sep;85(3):535-9. doi: 10.4269/ajtmh.2011.11-0037.
Abstract
Some protozoan infections such as Toxoplasma, Cryptosporidium, and Plasmodium can be transmitted through stem cell transplantations. To our knowledge, so far, there is no study about transmission of Leishmania parasites in stem cell transplantation and interactions between parasites and stem cells in vitro. Therefore, the aim of this study was to investigate the interaction between different species of Leishmania parasites and adipose tissue-derived mesenchymal stem cells (ADMSCs). ADMSCs have been isolated, cultured, characterized, and infected with different species of Leishmania parasites (L. donovani, L. major, L. tropica, and L. infantum). Infectivity was examined by Giemsa staining, microculture, and polymerase chain reaction methods. As a result, infectivity of ADMSCs by Leishmania parasites has been determined for the first time in this study. According to our findings, it is very important that donors are screened for Leishmania parasites before stem cell transplantations in regions where leishmaniasis is endemic.
摘要
一些原生动物感染，如弓形虫、隐孢子虫和疟原虫，可以通过干细胞移植传播。据我们所知，到目前为止，还没有关于干细胞移植中利什曼原虫寄生虫传播以及寄生虫和体外干细胞之间相互作用的研究。因此，本研究的目的是研究不同种利什曼原虫寄生虫与脂肪组织来源的间充质干细胞（ADMSCs）之间的相互作用。ADMSCs 已被分离、培养、鉴定，并感染了不同种利什曼原虫寄生虫（L. donovani、L. major、L. tropica 和 L. infantum）。通过吉姆萨染色、微培养和聚合酶链反应方法检查感染性。结果，本研究首次确定了 ADMSCs 被利什曼原虫寄生虫感染的能力。根据我们的发现，在利什曼病流行地区进行干细胞移植前，对供体进行利什曼原虫寄生虫筛查非常重要。
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