Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, UP, India.
Int J Neurosci. 2012 Dec;122(12):704-9. doi: 10.3109/00207454.2012.709893. Epub 2012 Aug 8.
In the present study, the effect of l-ascorbic acid (AA) was studied on the climbing ability of the Parkinson's disease (PD) model Drosophila expressing normal human alpha synuclein (h-αs) in the neurons. These flies show locomotor dysfunction as the age progresses. AA at final concentration of 11.35 × 10(-5) M, 22.71 × 10(-5) M, 45.42 × 10(-5) M, and 68.13 × 10(-5) M was added to the diet, and the flies were allowed to feed for 21 days. AA at 11.35 × 10(-5) M did not show any significant delay in the loss of climbing ability of PD model flies. However, AA at 22.71 × 10(-5) M, 45.42 × 10(-5) M, and 68.13 × 10(-5) M showed a dose dependent significant (p < .05) delay in the loss of climbing ability of PD model flies as compared to the untreated PD flies. The total protein concentration in brain homogenate was measured in treated as well as control groups after 21 days, no significant difference was obtained between treated as well as control (PD flies and l-dopa) groups. The results suggest that AA is potent in delaying the climbing disability of the PD model flies expressing h-αs in the neurons.
在本研究中，研究了抗坏血酸（AA）对表达正常人类α-突触核蛋白（h-αs）的神经元帕金森病（PD）模型果蝇攀爬能力的影响。这些果蝇随着年龄的增长表现出运动功能障碍。将终浓度为 11.35×10（-5）M、22.71×10（-5）M、45.42×10（-5）M 和 68.13×10（-5）M 的 AA 添加到饮食中，让果蝇进食 21 天。AA 在 11.35×10（-5）M 时，对 PD 模型果蝇失去攀爬能力的速度没有明显延缓作用。然而，AA 在 22.71×10（-5）M、45.42×10（-5）M 和 68.13×10（-5）M 时，与未处理的 PD 果蝇相比，显著延缓了 PD 模型果蝇失去攀爬能力的速度（p<.05）。在第 21 天，测量了处理组和对照组脑匀浆中的总蛋白浓度，在处理组和对照组（PD 果蝇和左旋多巴组）之间没有得到显著差异。结果表明，AA 能有效延缓表达 h-αs 的神经元 PD 模型果蝇的攀爬障碍。
